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Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru

Etiologic studies of acute febrile disease were conducted in sites across South America, including Cusco and Iquitos, Peru. Patients’ clinical signs and symptoms were recorded, and acute- and convalescent-phase serum samples were obtained for serologic examination and virus isolation in Vero E6 and...

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Autores principales: Oberste, M. Steven, Gotuzzo, Eduardo, Blair, Patrick, Nix, W. Allan, Ksiazek, Thomas G., Comer, James A., Rollin, Pierre, Goldsmith, Cynthia S., Olson, James, Kochel, Tadeusz J.
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2671410/
https://www.ncbi.nlm.nih.gov/pubmed/19331761
http://dx.doi.org/10.3201/eid1504.081428
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author Oberste, M. Steven
Gotuzzo, Eduardo
Blair, Patrick
Nix, W. Allan
Ksiazek, Thomas G.
Comer, James A.
Rollin, Pierre
Goldsmith, Cynthia S.
Olson, James
Kochel, Tadeusz J.
author_facet Oberste, M. Steven
Gotuzzo, Eduardo
Blair, Patrick
Nix, W. Allan
Ksiazek, Thomas G.
Comer, James A.
Rollin, Pierre
Goldsmith, Cynthia S.
Olson, James
Kochel, Tadeusz J.
author_sort Oberste, M. Steven
collection PubMed
description Etiologic studies of acute febrile disease were conducted in sites across South America, including Cusco and Iquitos, Peru. Patients’ clinical signs and symptoms were recorded, and acute- and convalescent-phase serum samples were obtained for serologic examination and virus isolation in Vero E6 and C6/36 cells. Virus isolated in Vero E6 cells was identified as encephalomyocarditis virus (EMCV) by electron microscopy and by subsequent molecular diagnostic testing of samples from 2 febrile patients with nausea, headache, and dyspnea. The virus was recovered from acute-phase serum samples from both case-patients and identified with cardiovirus-specific reverse transcription–PCR and sequencing. Serum samples from case-patient 1 showed cardiovirus antibody by immunoglobulin M ELISA (acute phase <8, convalescent phase >1,024) and by neutralization assay (acute phase <10, convalescent phase >1,280). Serum samples from case-patient 2 did not contain antibodies detectable by either assay. Detection of virus in serum strongly supports a role for EMCV in human infection and febrile illness.
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spelling pubmed-26714102009-05-21 Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru Oberste, M. Steven Gotuzzo, Eduardo Blair, Patrick Nix, W. Allan Ksiazek, Thomas G. Comer, James A. Rollin, Pierre Goldsmith, Cynthia S. Olson, James Kochel, Tadeusz J. Emerg Infect Dis Research Etiologic studies of acute febrile disease were conducted in sites across South America, including Cusco and Iquitos, Peru. Patients’ clinical signs and symptoms were recorded, and acute- and convalescent-phase serum samples were obtained for serologic examination and virus isolation in Vero E6 and C6/36 cells. Virus isolated in Vero E6 cells was identified as encephalomyocarditis virus (EMCV) by electron microscopy and by subsequent molecular diagnostic testing of samples from 2 febrile patients with nausea, headache, and dyspnea. The virus was recovered from acute-phase serum samples from both case-patients and identified with cardiovirus-specific reverse transcription–PCR and sequencing. Serum samples from case-patient 1 showed cardiovirus antibody by immunoglobulin M ELISA (acute phase <8, convalescent phase >1,024) and by neutralization assay (acute phase <10, convalescent phase >1,280). Serum samples from case-patient 2 did not contain antibodies detectable by either assay. Detection of virus in serum strongly supports a role for EMCV in human infection and febrile illness. Centers for Disease Control and Prevention 2009-04 /pmc/articles/PMC2671410/ /pubmed/19331761 http://dx.doi.org/10.3201/eid1504.081428 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Oberste, M. Steven
Gotuzzo, Eduardo
Blair, Patrick
Nix, W. Allan
Ksiazek, Thomas G.
Comer, James A.
Rollin, Pierre
Goldsmith, Cynthia S.
Olson, James
Kochel, Tadeusz J.
Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru
title Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru
title_full Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru
title_fullStr Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru
title_full_unstemmed Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru
title_short Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru
title_sort human febrile illness caused by encephalomyocarditis virus infection, peru
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2671410/
https://www.ncbi.nlm.nih.gov/pubmed/19331761
http://dx.doi.org/10.3201/eid1504.081428
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