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Strong Dietary Restrictions Protect Drosophila against Anoxia/Reoxygenation Injuries
BACKGROUND: Reoxygenation of ischemic tissues is a major factor that determines the severity of cardiovascular diseases. This paper describes the consequences of anoxia/reoxygenation (A/R) stresses on Drosophila, a useful, anoxia tolerant, model organism. METHODOLOGY/PRINCIPAL FINDINGS: Newly emerge...
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2671842/ https://www.ncbi.nlm.nih.gov/pubmed/19412543 http://dx.doi.org/10.1371/journal.pone.0005422 |
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author | Vigne, Paul Tauc, Michel Frelin, Christian |
author_facet | Vigne, Paul Tauc, Michel Frelin, Christian |
author_sort | Vigne, Paul |
collection | PubMed |
description | BACKGROUND: Reoxygenation of ischemic tissues is a major factor that determines the severity of cardiovascular diseases. This paper describes the consequences of anoxia/reoxygenation (A/R) stresses on Drosophila, a useful, anoxia tolerant, model organism. METHODOLOGY/PRINCIPAL FINDINGS: Newly emerged adult male flies were exposed to anoxic conditions (<1% O(2)) for 1 to 6 hours, reoxygenated and their survival was monitored. RESULTS: A/R stresses induced a transient increase in mortality which peaked at the time of reoxygenation. Then flies recovered low mortality rates similar to those of control flies. A/R induced mortality was strongly dependent on dietary conditions during the 48 h that preceded anoxia. Well fed flies were anoxia sensitive. Strong dietary restrictions and starvation conditions protected flies against A/R injuries. The tolerance to anoxia was associated to large decreases in glycogen, protein, and ATP contents. During anoxia, anoxia tolerant flies produced more lactate, less phosphate and they maintained more stable ATP levels than anoxia sensitive flies. Moderate dietary restrictions, which increased the longevity of normoxic flies, did not promote resistance to A/R stresses. Diet dependent A/R injuries were still observed in sima loss of function mutants and they were insensitive to dietary rapamycin or resveratrol. AICAR (5-aminoimidazole-4-carboxamide-1-beta-D-ribosefuranoside), an activator AMP kinase decreased A/R injuries. Mutants in the insulin signalling pathway were more anoxia tolerant in a fed state. CONCLUSION/SIGNIFICANCE: Long A/R stresses induce a transient increase in mortality in Drosophila. This mortality is highly dependent on dietary conditions prior to the stress. Strong dietary restrictions and starvation conditions protect flies against A/R injuries, probably by inducing a major remodelling of energy metabolism. The results also indicate that mechanistically different responses develop in response to dietary restrictions of different strengths. AMP kinase and the insulin signalling pathway are possible mediators of diet dependent anoxic tolerance in Drosophila. |
format | Text |
id | pubmed-2671842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26718422009-05-01 Strong Dietary Restrictions Protect Drosophila against Anoxia/Reoxygenation Injuries Vigne, Paul Tauc, Michel Frelin, Christian PLoS One Research Article BACKGROUND: Reoxygenation of ischemic tissues is a major factor that determines the severity of cardiovascular diseases. This paper describes the consequences of anoxia/reoxygenation (A/R) stresses on Drosophila, a useful, anoxia tolerant, model organism. METHODOLOGY/PRINCIPAL FINDINGS: Newly emerged adult male flies were exposed to anoxic conditions (<1% O(2)) for 1 to 6 hours, reoxygenated and their survival was monitored. RESULTS: A/R stresses induced a transient increase in mortality which peaked at the time of reoxygenation. Then flies recovered low mortality rates similar to those of control flies. A/R induced mortality was strongly dependent on dietary conditions during the 48 h that preceded anoxia. Well fed flies were anoxia sensitive. Strong dietary restrictions and starvation conditions protected flies against A/R injuries. The tolerance to anoxia was associated to large decreases in glycogen, protein, and ATP contents. During anoxia, anoxia tolerant flies produced more lactate, less phosphate and they maintained more stable ATP levels than anoxia sensitive flies. Moderate dietary restrictions, which increased the longevity of normoxic flies, did not promote resistance to A/R stresses. Diet dependent A/R injuries were still observed in sima loss of function mutants and they were insensitive to dietary rapamycin or resveratrol. AICAR (5-aminoimidazole-4-carboxamide-1-beta-D-ribosefuranoside), an activator AMP kinase decreased A/R injuries. Mutants in the insulin signalling pathway were more anoxia tolerant in a fed state. CONCLUSION/SIGNIFICANCE: Long A/R stresses induce a transient increase in mortality in Drosophila. This mortality is highly dependent on dietary conditions prior to the stress. Strong dietary restrictions and starvation conditions protect flies against A/R injuries, probably by inducing a major remodelling of energy metabolism. The results also indicate that mechanistically different responses develop in response to dietary restrictions of different strengths. AMP kinase and the insulin signalling pathway are possible mediators of diet dependent anoxic tolerance in Drosophila. Public Library of Science 2009-05-01 /pmc/articles/PMC2671842/ /pubmed/19412543 http://dx.doi.org/10.1371/journal.pone.0005422 Text en Vigne et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Vigne, Paul Tauc, Michel Frelin, Christian Strong Dietary Restrictions Protect Drosophila against Anoxia/Reoxygenation Injuries |
title | Strong Dietary Restrictions Protect Drosophila against Anoxia/Reoxygenation Injuries |
title_full | Strong Dietary Restrictions Protect Drosophila against Anoxia/Reoxygenation Injuries |
title_fullStr | Strong Dietary Restrictions Protect Drosophila against Anoxia/Reoxygenation Injuries |
title_full_unstemmed | Strong Dietary Restrictions Protect Drosophila against Anoxia/Reoxygenation Injuries |
title_short | Strong Dietary Restrictions Protect Drosophila against Anoxia/Reoxygenation Injuries |
title_sort | strong dietary restrictions protect drosophila against anoxia/reoxygenation injuries |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2671842/ https://www.ncbi.nlm.nih.gov/pubmed/19412543 http://dx.doi.org/10.1371/journal.pone.0005422 |
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