Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model

We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (...

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Autores principales: Rakwal, Randeep, Agrawal, Ganesh Kumar, Shibato, Junko, Imanaka, Tetsuji, Fukutani, Satoshi, Tamogami, Shigeru, Endo, Satoru, Sahoo, Sarata Kumar, Masuo, Yoshinori, Kimura, Shinzo
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2672026/
https://www.ncbi.nlm.nih.gov/pubmed/19399245
http://dx.doi.org/10.3390/ijms10031215
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author Rakwal, Randeep
Agrawal, Ganesh Kumar
Shibato, Junko
Imanaka, Tetsuji
Fukutani, Satoshi
Tamogami, Shigeru
Endo, Satoru
Sahoo, Sarata Kumar
Masuo, Yoshinori
Kimura, Shinzo
author_facet Rakwal, Randeep
Agrawal, Ganesh Kumar
Shibato, Junko
Imanaka, Tetsuji
Fukutani, Satoshi
Tamogami, Shigeru
Endo, Satoru
Sahoo, Sarata Kumar
Masuo, Yoshinori
Kimura, Shinzo
author_sort Rakwal, Randeep
collection PubMed
description We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (γ) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s.
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spelling pubmed-26720262009-04-27 Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model Rakwal, Randeep Agrawal, Ganesh Kumar Shibato, Junko Imanaka, Tetsuji Fukutani, Satoshi Tamogami, Shigeru Endo, Satoru Sahoo, Sarata Kumar Masuo, Yoshinori Kimura, Shinzo Int J Mol Sci Article We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (γ) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s. Molecular Diversity Preservation International (MDPI) 2009-03-16 /pmc/articles/PMC2672026/ /pubmed/19399245 http://dx.doi.org/10.3390/ijms10031215 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Rakwal, Randeep
Agrawal, Ganesh Kumar
Shibato, Junko
Imanaka, Tetsuji
Fukutani, Satoshi
Tamogami, Shigeru
Endo, Satoru
Sahoo, Sarata Kumar
Masuo, Yoshinori
Kimura, Shinzo
Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model
title Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model
title_full Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model
title_fullStr Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model
title_full_unstemmed Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model
title_short Ultra Low-Dose Radiation: Stress Responses and Impacts Using Rice as a Grass Model
title_sort ultra low-dose radiation: stress responses and impacts using rice as a grass model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2672026/
https://www.ncbi.nlm.nih.gov/pubmed/19399245
http://dx.doi.org/10.3390/ijms10031215
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