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Transcriptional Network of p63 in Human Keratinocytes
p63 is a transcription factor required for the development and maintenance of ectodermal tissues in general, and skin keratinocytes in particular. The identification of its target genes is fundamental for understanding the complex network of gene regulation governing the development of epithelia. We...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2672041/ https://www.ncbi.nlm.nih.gov/pubmed/19390658 http://dx.doi.org/10.1371/journal.pone.0005008 |
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author | Pozzi, Silvia Zambelli, Federico Merico, Daniele Pavesi, Giulio Robert, Amélie Maltère, Peggy Gidrol, Xavier Mantovani, Roberto Vigano, M. Alessandra |
author_facet | Pozzi, Silvia Zambelli, Federico Merico, Daniele Pavesi, Giulio Robert, Amélie Maltère, Peggy Gidrol, Xavier Mantovani, Roberto Vigano, M. Alessandra |
author_sort | Pozzi, Silvia |
collection | PubMed |
description | p63 is a transcription factor required for the development and maintenance of ectodermal tissues in general, and skin keratinocytes in particular. The identification of its target genes is fundamental for understanding the complex network of gene regulation governing the development of epithelia. We report a list of almost 1000 targets derived from ChIP on chip analysis on two platforms; all genes analyzed changed in expression during differentiation of human keratinocytes. Functional annotation highlighted unexpected GO terms enrichments and confirmed that genes involved in transcriptional regulation are the most significant. A detailed analysis of these transcriptional regulators in condition of perturbed p63 levels confirmed the role of p63 in the regulatory network. Rather than a rigid master-slave hierarchical model, our data indicate that p63 connects different hubs involved in the multiple specific functions of the skin. |
format | Text |
id | pubmed-2672041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26720412009-04-23 Transcriptional Network of p63 in Human Keratinocytes Pozzi, Silvia Zambelli, Federico Merico, Daniele Pavesi, Giulio Robert, Amélie Maltère, Peggy Gidrol, Xavier Mantovani, Roberto Vigano, M. Alessandra PLoS One Research Article p63 is a transcription factor required for the development and maintenance of ectodermal tissues in general, and skin keratinocytes in particular. The identification of its target genes is fundamental for understanding the complex network of gene regulation governing the development of epithelia. We report a list of almost 1000 targets derived from ChIP on chip analysis on two platforms; all genes analyzed changed in expression during differentiation of human keratinocytes. Functional annotation highlighted unexpected GO terms enrichments and confirmed that genes involved in transcriptional regulation are the most significant. A detailed analysis of these transcriptional regulators in condition of perturbed p63 levels confirmed the role of p63 in the regulatory network. Rather than a rigid master-slave hierarchical model, our data indicate that p63 connects different hubs involved in the multiple specific functions of the skin. Public Library of Science 2009-03-25 /pmc/articles/PMC2672041/ /pubmed/19390658 http://dx.doi.org/10.1371/journal.pone.0005008 Text en Pozzi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Pozzi, Silvia Zambelli, Federico Merico, Daniele Pavesi, Giulio Robert, Amélie Maltère, Peggy Gidrol, Xavier Mantovani, Roberto Vigano, M. Alessandra Transcriptional Network of p63 in Human Keratinocytes |
title | Transcriptional Network of p63 in Human Keratinocytes |
title_full | Transcriptional Network of p63 in Human Keratinocytes |
title_fullStr | Transcriptional Network of p63 in Human Keratinocytes |
title_full_unstemmed | Transcriptional Network of p63 in Human Keratinocytes |
title_short | Transcriptional Network of p63 in Human Keratinocytes |
title_sort | transcriptional network of p63 in human keratinocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2672041/ https://www.ncbi.nlm.nih.gov/pubmed/19390658 http://dx.doi.org/10.1371/journal.pone.0005008 |
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