Cargando…

Transcriptional Network of p63 in Human Keratinocytes

p63 is a transcription factor required for the development and maintenance of ectodermal tissues in general, and skin keratinocytes in particular. The identification of its target genes is fundamental for understanding the complex network of gene regulation governing the development of epithelia. We...

Descripción completa

Detalles Bibliográficos
Autores principales: Pozzi, Silvia, Zambelli, Federico, Merico, Daniele, Pavesi, Giulio, Robert, Amélie, Maltère, Peggy, Gidrol, Xavier, Mantovani, Roberto, Vigano, M. Alessandra
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2672041/
https://www.ncbi.nlm.nih.gov/pubmed/19390658
http://dx.doi.org/10.1371/journal.pone.0005008
_version_ 1782166462631247872
author Pozzi, Silvia
Zambelli, Federico
Merico, Daniele
Pavesi, Giulio
Robert, Amélie
Maltère, Peggy
Gidrol, Xavier
Mantovani, Roberto
Vigano, M. Alessandra
author_facet Pozzi, Silvia
Zambelli, Federico
Merico, Daniele
Pavesi, Giulio
Robert, Amélie
Maltère, Peggy
Gidrol, Xavier
Mantovani, Roberto
Vigano, M. Alessandra
author_sort Pozzi, Silvia
collection PubMed
description p63 is a transcription factor required for the development and maintenance of ectodermal tissues in general, and skin keratinocytes in particular. The identification of its target genes is fundamental for understanding the complex network of gene regulation governing the development of epithelia. We report a list of almost 1000 targets derived from ChIP on chip analysis on two platforms; all genes analyzed changed in expression during differentiation of human keratinocytes. Functional annotation highlighted unexpected GO terms enrichments and confirmed that genes involved in transcriptional regulation are the most significant. A detailed analysis of these transcriptional regulators in condition of perturbed p63 levels confirmed the role of p63 in the regulatory network. Rather than a rigid master-slave hierarchical model, our data indicate that p63 connects different hubs involved in the multiple specific functions of the skin.
format Text
id pubmed-2672041
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-26720412009-04-23 Transcriptional Network of p63 in Human Keratinocytes Pozzi, Silvia Zambelli, Federico Merico, Daniele Pavesi, Giulio Robert, Amélie Maltère, Peggy Gidrol, Xavier Mantovani, Roberto Vigano, M. Alessandra PLoS One Research Article p63 is a transcription factor required for the development and maintenance of ectodermal tissues in general, and skin keratinocytes in particular. The identification of its target genes is fundamental for understanding the complex network of gene regulation governing the development of epithelia. We report a list of almost 1000 targets derived from ChIP on chip analysis on two platforms; all genes analyzed changed in expression during differentiation of human keratinocytes. Functional annotation highlighted unexpected GO terms enrichments and confirmed that genes involved in transcriptional regulation are the most significant. A detailed analysis of these transcriptional regulators in condition of perturbed p63 levels confirmed the role of p63 in the regulatory network. Rather than a rigid master-slave hierarchical model, our data indicate that p63 connects different hubs involved in the multiple specific functions of the skin. Public Library of Science 2009-03-25 /pmc/articles/PMC2672041/ /pubmed/19390658 http://dx.doi.org/10.1371/journal.pone.0005008 Text en Pozzi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pozzi, Silvia
Zambelli, Federico
Merico, Daniele
Pavesi, Giulio
Robert, Amélie
Maltère, Peggy
Gidrol, Xavier
Mantovani, Roberto
Vigano, M. Alessandra
Transcriptional Network of p63 in Human Keratinocytes
title Transcriptional Network of p63 in Human Keratinocytes
title_full Transcriptional Network of p63 in Human Keratinocytes
title_fullStr Transcriptional Network of p63 in Human Keratinocytes
title_full_unstemmed Transcriptional Network of p63 in Human Keratinocytes
title_short Transcriptional Network of p63 in Human Keratinocytes
title_sort transcriptional network of p63 in human keratinocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2672041/
https://www.ncbi.nlm.nih.gov/pubmed/19390658
http://dx.doi.org/10.1371/journal.pone.0005008
work_keys_str_mv AT pozzisilvia transcriptionalnetworkofp63inhumankeratinocytes
AT zambellifederico transcriptionalnetworkofp63inhumankeratinocytes
AT mericodaniele transcriptionalnetworkofp63inhumankeratinocytes
AT pavesigiulio transcriptionalnetworkofp63inhumankeratinocytes
AT robertamelie transcriptionalnetworkofp63inhumankeratinocytes
AT malterepeggy transcriptionalnetworkofp63inhumankeratinocytes
AT gidrolxavier transcriptionalnetworkofp63inhumankeratinocytes
AT mantovaniroberto transcriptionalnetworkofp63inhumankeratinocytes
AT viganomalessandra transcriptionalnetworkofp63inhumankeratinocytes