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Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo
MicroRNAs (miRs) are involved in key biological processes via suppression of gene expression at posttranscriptional levels. According to their superior functions, subtle modulation of miR expression by certain compounds or nutrients is desirable under particular conditions. Bacterial lipopolysacchar...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2672161/ https://www.ncbi.nlm.nih.gov/pubmed/19390647 http://dx.doi.org/10.1155/2009/415437 |
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author | Schmelzer, Constance Kitano, Mitsuaki Rimbach, Gerald Niklowitz, Petra Menke, Thomas Hosoe, Kazunori Döring, Frank |
author_facet | Schmelzer, Constance Kitano, Mitsuaki Rimbach, Gerald Niklowitz, Petra Menke, Thomas Hosoe, Kazunori Döring, Frank |
author_sort | Schmelzer, Constance |
collection | PubMed |
description | MicroRNAs (miRs) are involved in key biological processes via suppression of gene expression at posttranscriptional levels. According to their superior functions, subtle modulation of miR expression by certain compounds or nutrients is desirable under particular conditions. Bacterial lipopolysaccharide (LPS) induces a reactive oxygen species-/NF-κB-dependent pathway which increases the expression of the anti-inflammatory miR-146a. We hypothesized that this induction could be modulated by the antioxidant ubiquinol-10. Preincubation of human monocytic THP-1 cells with ubiquinol-10 reduced the LPS-induced expression level of miR-146a to 78.9 ± 13.22%. In liver samples of mice injected with LPS, supplementation with ubiquinol-10 leads to a reduction of LPS-induced miR-146a expression to 78.12 ± 21.25%. From these consistent in vitro and in vivo data, we conclude that ubiquinol-10 may fine-tune the inflammatory response via moderate reduction of miR-146a expression. |
format | Text |
id | pubmed-2672161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-26721612009-04-23 Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo Schmelzer, Constance Kitano, Mitsuaki Rimbach, Gerald Niklowitz, Petra Menke, Thomas Hosoe, Kazunori Döring, Frank Mediators Inflamm Research Article MicroRNAs (miRs) are involved in key biological processes via suppression of gene expression at posttranscriptional levels. According to their superior functions, subtle modulation of miR expression by certain compounds or nutrients is desirable under particular conditions. Bacterial lipopolysaccharide (LPS) induces a reactive oxygen species-/NF-κB-dependent pathway which increases the expression of the anti-inflammatory miR-146a. We hypothesized that this induction could be modulated by the antioxidant ubiquinol-10. Preincubation of human monocytic THP-1 cells with ubiquinol-10 reduced the LPS-induced expression level of miR-146a to 78.9 ± 13.22%. In liver samples of mice injected with LPS, supplementation with ubiquinol-10 leads to a reduction of LPS-induced miR-146a expression to 78.12 ± 21.25%. From these consistent in vitro and in vivo data, we conclude that ubiquinol-10 may fine-tune the inflammatory response via moderate reduction of miR-146a expression. Hindawi Publishing Corporation 2009 2009-04-16 /pmc/articles/PMC2672161/ /pubmed/19390647 http://dx.doi.org/10.1155/2009/415437 Text en Copyright © 2009 Constance Schmelzer et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Schmelzer, Constance Kitano, Mitsuaki Rimbach, Gerald Niklowitz, Petra Menke, Thomas Hosoe, Kazunori Döring, Frank Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
title | Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
title_full | Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
title_fullStr | Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
title_full_unstemmed | Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
title_short | Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
title_sort | effects of ubiquinol-10 on microrna-146a expression in vitro and in vivo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2672161/ https://www.ncbi.nlm.nih.gov/pubmed/19390647 http://dx.doi.org/10.1155/2009/415437 |
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