Cargando…

Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells

BACKGROUND: RhoA and RhoC are deregulated by over expression in many human tumors, including colorectal cancer. Some reports show that they play a pivotal role in the carcinogenesis, tumor development and infiltration metastasis. In this study, for the first time we constructed recombinant adenoviru...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xiang-ping, Wang, Hai-bo, Yang, Kun, Sui, Ai-hua, Shi, Qiang, Qu, Shen
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2672922/
https://www.ncbi.nlm.nih.gov/pubmed/19374769
http://dx.doi.org/10.1186/1756-9966-28-52
_version_ 1782166554921664512
author Liu, Xiang-ping
Wang, Hai-bo
Yang, Kun
Sui, Ai-hua
Shi, Qiang
Qu, Shen
author_facet Liu, Xiang-ping
Wang, Hai-bo
Yang, Kun
Sui, Ai-hua
Shi, Qiang
Qu, Shen
author_sort Liu, Xiang-ping
collection PubMed
description BACKGROUND: RhoA and RhoC are deregulated by over expression in many human tumors, including colorectal cancer. Some reports show that they play a pivotal role in the carcinogenesis, tumor development and infiltration metastasis. In this study, for the first time we constructed recombinant adenovirus to investigate the inhibitory effects of RhoA and RhoC shRNAs in tandem expression on the cell proliferation and invasion of colorectal cancer HCT116 cells. METHODS: The recombinant adenovirus carrying RhoA and RhoC shRNAs in tandem expression was transfected into HCT116. The mRNA transcription and protein expressions of RhoA and RhoC were examined by RT-FQPCR and Western blot respectively. Cellular proliferation inhibitory activity was determined by methyl thiazolyl tetrazolium (MTT) assay and invasive and migrating potential was detected through in vitro Matrigel coated invasion and migration assay. RESULTS: Both mRNA and proteins Levels of RhoA and RhoC were significantly reduced in HCT116 cells transfected with Ad-A1+A2+C1+C2 than those in Ad-HK group and control one. The relative RhoA and RhoC mRNA transcriptions were decreased to 40% and 36% (P < 0.05), while proteins expression reducing 42% and 35%, respectively (P < 0.05). Growth curves analysis showed that alive cell number in the Ad-A1+A2+C1+C2 group was lower than others in the third to sixth day and transwell chamber analysis showed that migration/invasion activity was significantly suppressed in Ad-A1+A2+C1+C2 group. CONCLUSION: Our results indicate recombinant adenovirus carrying RhoA and RhoC shRNAs in tandem expression may inhibit the growth and invasion of HCT116 cells. Application of such vector to inhibit one or more genes may be a new method to cancer therapy.
format Text
id pubmed-2672922
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-26729222009-04-24 Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells Liu, Xiang-ping Wang, Hai-bo Yang, Kun Sui, Ai-hua Shi, Qiang Qu, Shen J Exp Clin Cancer Res Research BACKGROUND: RhoA and RhoC are deregulated by over expression in many human tumors, including colorectal cancer. Some reports show that they play a pivotal role in the carcinogenesis, tumor development and infiltration metastasis. In this study, for the first time we constructed recombinant adenovirus to investigate the inhibitory effects of RhoA and RhoC shRNAs in tandem expression on the cell proliferation and invasion of colorectal cancer HCT116 cells. METHODS: The recombinant adenovirus carrying RhoA and RhoC shRNAs in tandem expression was transfected into HCT116. The mRNA transcription and protein expressions of RhoA and RhoC were examined by RT-FQPCR and Western blot respectively. Cellular proliferation inhibitory activity was determined by methyl thiazolyl tetrazolium (MTT) assay and invasive and migrating potential was detected through in vitro Matrigel coated invasion and migration assay. RESULTS: Both mRNA and proteins Levels of RhoA and RhoC were significantly reduced in HCT116 cells transfected with Ad-A1+A2+C1+C2 than those in Ad-HK group and control one. The relative RhoA and RhoC mRNA transcriptions were decreased to 40% and 36% (P < 0.05), while proteins expression reducing 42% and 35%, respectively (P < 0.05). Growth curves analysis showed that alive cell number in the Ad-A1+A2+C1+C2 group was lower than others in the third to sixth day and transwell chamber analysis showed that migration/invasion activity was significantly suppressed in Ad-A1+A2+C1+C2 group. CONCLUSION: Our results indicate recombinant adenovirus carrying RhoA and RhoC shRNAs in tandem expression may inhibit the growth and invasion of HCT116 cells. Application of such vector to inhibit one or more genes may be a new method to cancer therapy. BioMed Central 2009-04-18 /pmc/articles/PMC2672922/ /pubmed/19374769 http://dx.doi.org/10.1186/1756-9966-28-52 Text en Copyright © 2009 Liu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Liu, Xiang-ping
Wang, Hai-bo
Yang, Kun
Sui, Ai-hua
Shi, Qiang
Qu, Shen
Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells
title Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells
title_full Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells
title_fullStr Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells
title_full_unstemmed Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells
title_short Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells
title_sort inhibitory effects of adenovirus mediated tandem expression of rhoa and rhoc shrnas in hct116 cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2672922/
https://www.ncbi.nlm.nih.gov/pubmed/19374769
http://dx.doi.org/10.1186/1756-9966-28-52
work_keys_str_mv AT liuxiangping inhibitoryeffectsofadenovirusmediatedtandemexpressionofrhoaandrhocshrnasinhct116cells
AT wanghaibo inhibitoryeffectsofadenovirusmediatedtandemexpressionofrhoaandrhocshrnasinhct116cells
AT yangkun inhibitoryeffectsofadenovirusmediatedtandemexpressionofrhoaandrhocshrnasinhct116cells
AT suiaihua inhibitoryeffectsofadenovirusmediatedtandemexpressionofrhoaandrhocshrnasinhct116cells
AT shiqiang inhibitoryeffectsofadenovirusmediatedtandemexpressionofrhoaandrhocshrnasinhct116cells
AT qushen inhibitoryeffectsofadenovirusmediatedtandemexpressionofrhoaandrhocshrnasinhct116cells