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Proteomic study on gender differences in aging kidney of mice

BACKGROUND: This study aims to analyze sex differences in mice aging kidney. We applied a proteomic technique based on subfractionation, and liquid chromatography coupled with 2-DE. Samples from male and female CD1-Swiss outbred mice from 28 weeks, 52 weeks, and 76 weeks were analysed by 2-DE, and s...

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Autores principales: Amelina, Hanna, Cristobal, Susana
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673210/
https://www.ncbi.nlm.nih.gov/pubmed/19358702
http://dx.doi.org/10.1186/1477-5956-7-16
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author Amelina, Hanna
Cristobal, Susana
author_facet Amelina, Hanna
Cristobal, Susana
author_sort Amelina, Hanna
collection PubMed
description BACKGROUND: This study aims to analyze sex differences in mice aging kidney. We applied a proteomic technique based on subfractionation, and liquid chromatography coupled with 2-DE. Samples from male and female CD1-Swiss outbred mice from 28 weeks, 52 weeks, and 76 weeks were analysed by 2-DE, and selected proteins were identified by matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). RESULTS: This proteomic analysis detected age-related changes in protein expression in 55 protein-spots, corresponding to 22 spots in males and 33 spots in females. We found a protein expression signature (PES) of aging composed by 8 spots, common for both genders. The identified proteins indicated increases in oxidative and proteolytic proteins and decreases in glycolytic proteins, and antioxidant enzymes. CONCLUSION: Our results provide insights into the gender differences associated to the decline of kidney function in aging. Thus, we show that proteomics can provide valuable information on age-related changes in expression levels of proteins and related modifications. This pilot study is still far from providing candidates for aging-biomarkers. However, we suggest that the analysis of these proteins could suggest mechanisms of cellular aging in kidney, and improve the kidney selection for transplantation.
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spelling pubmed-26732102009-04-25 Proteomic study on gender differences in aging kidney of mice Amelina, Hanna Cristobal, Susana Proteome Sci Research BACKGROUND: This study aims to analyze sex differences in mice aging kidney. We applied a proteomic technique based on subfractionation, and liquid chromatography coupled with 2-DE. Samples from male and female CD1-Swiss outbred mice from 28 weeks, 52 weeks, and 76 weeks were analysed by 2-DE, and selected proteins were identified by matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). RESULTS: This proteomic analysis detected age-related changes in protein expression in 55 protein-spots, corresponding to 22 spots in males and 33 spots in females. We found a protein expression signature (PES) of aging composed by 8 spots, common for both genders. The identified proteins indicated increases in oxidative and proteolytic proteins and decreases in glycolytic proteins, and antioxidant enzymes. CONCLUSION: Our results provide insights into the gender differences associated to the decline of kidney function in aging. Thus, we show that proteomics can provide valuable information on age-related changes in expression levels of proteins and related modifications. This pilot study is still far from providing candidates for aging-biomarkers. However, we suggest that the analysis of these proteins could suggest mechanisms of cellular aging in kidney, and improve the kidney selection for transplantation. BioMed Central 2009-04-09 /pmc/articles/PMC2673210/ /pubmed/19358702 http://dx.doi.org/10.1186/1477-5956-7-16 Text en Copyright © 2009 Amelina and Cristobal; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Amelina, Hanna
Cristobal, Susana
Proteomic study on gender differences in aging kidney of mice
title Proteomic study on gender differences in aging kidney of mice
title_full Proteomic study on gender differences in aging kidney of mice
title_fullStr Proteomic study on gender differences in aging kidney of mice
title_full_unstemmed Proteomic study on gender differences in aging kidney of mice
title_short Proteomic study on gender differences in aging kidney of mice
title_sort proteomic study on gender differences in aging kidney of mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673210/
https://www.ncbi.nlm.nih.gov/pubmed/19358702
http://dx.doi.org/10.1186/1477-5956-7-16
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