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Proteomic study on gender differences in aging kidney of mice
BACKGROUND: This study aims to analyze sex differences in mice aging kidney. We applied a proteomic technique based on subfractionation, and liquid chromatography coupled with 2-DE. Samples from male and female CD1-Swiss outbred mice from 28 weeks, 52 weeks, and 76 weeks were analysed by 2-DE, and s...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673210/ https://www.ncbi.nlm.nih.gov/pubmed/19358702 http://dx.doi.org/10.1186/1477-5956-7-16 |
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author | Amelina, Hanna Cristobal, Susana |
author_facet | Amelina, Hanna Cristobal, Susana |
author_sort | Amelina, Hanna |
collection | PubMed |
description | BACKGROUND: This study aims to analyze sex differences in mice aging kidney. We applied a proteomic technique based on subfractionation, and liquid chromatography coupled with 2-DE. Samples from male and female CD1-Swiss outbred mice from 28 weeks, 52 weeks, and 76 weeks were analysed by 2-DE, and selected proteins were identified by matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). RESULTS: This proteomic analysis detected age-related changes in protein expression in 55 protein-spots, corresponding to 22 spots in males and 33 spots in females. We found a protein expression signature (PES) of aging composed by 8 spots, common for both genders. The identified proteins indicated increases in oxidative and proteolytic proteins and decreases in glycolytic proteins, and antioxidant enzymes. CONCLUSION: Our results provide insights into the gender differences associated to the decline of kidney function in aging. Thus, we show that proteomics can provide valuable information on age-related changes in expression levels of proteins and related modifications. This pilot study is still far from providing candidates for aging-biomarkers. However, we suggest that the analysis of these proteins could suggest mechanisms of cellular aging in kidney, and improve the kidney selection for transplantation. |
format | Text |
id | pubmed-2673210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26732102009-04-25 Proteomic study on gender differences in aging kidney of mice Amelina, Hanna Cristobal, Susana Proteome Sci Research BACKGROUND: This study aims to analyze sex differences in mice aging kidney. We applied a proteomic technique based on subfractionation, and liquid chromatography coupled with 2-DE. Samples from male and female CD1-Swiss outbred mice from 28 weeks, 52 weeks, and 76 weeks were analysed by 2-DE, and selected proteins were identified by matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). RESULTS: This proteomic analysis detected age-related changes in protein expression in 55 protein-spots, corresponding to 22 spots in males and 33 spots in females. We found a protein expression signature (PES) of aging composed by 8 spots, common for both genders. The identified proteins indicated increases in oxidative and proteolytic proteins and decreases in glycolytic proteins, and antioxidant enzymes. CONCLUSION: Our results provide insights into the gender differences associated to the decline of kidney function in aging. Thus, we show that proteomics can provide valuable information on age-related changes in expression levels of proteins and related modifications. This pilot study is still far from providing candidates for aging-biomarkers. However, we suggest that the analysis of these proteins could suggest mechanisms of cellular aging in kidney, and improve the kidney selection for transplantation. BioMed Central 2009-04-09 /pmc/articles/PMC2673210/ /pubmed/19358702 http://dx.doi.org/10.1186/1477-5956-7-16 Text en Copyright © 2009 Amelina and Cristobal; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Amelina, Hanna Cristobal, Susana Proteomic study on gender differences in aging kidney of mice |
title | Proteomic study on gender differences in aging kidney of mice |
title_full | Proteomic study on gender differences in aging kidney of mice |
title_fullStr | Proteomic study on gender differences in aging kidney of mice |
title_full_unstemmed | Proteomic study on gender differences in aging kidney of mice |
title_short | Proteomic study on gender differences in aging kidney of mice |
title_sort | proteomic study on gender differences in aging kidney of mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673210/ https://www.ncbi.nlm.nih.gov/pubmed/19358702 http://dx.doi.org/10.1186/1477-5956-7-16 |
work_keys_str_mv | AT amelinahanna proteomicstudyongenderdifferencesinagingkidneyofmice AT cristobalsusana proteomicstudyongenderdifferencesinagingkidneyofmice |