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Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae
PCR-mediated gene modification is a powerful approach to the functional analysis of genes in Saccharomyces cerevisiae. One application of this method is epitope-tagging of a gene to analyse the corresponding protein by immunological methods. However, the number of epitope tags available in a conveni...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley & Sons, Ltd.
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673350/ https://www.ncbi.nlm.nih.gov/pubmed/19243080 http://dx.doi.org/10.1002/yea.1658 |