The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication

The GINS complex, originally discovered in Saccharomyces cerevisiae and Xenopus laevis, binds to DNA replication origins shortly before the onset of S phase and travels with the replication forks after initiation. In this study we present a detailed characterization of the human GINS (hGINS) homolog...

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Autores principales: Aparicio, Tomás, Guillou, Emmanuelle, Coloma, Javier, Montoya, Guillermo, Méndez, Juan
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Genome Integrity, Repair and Replication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673421/
https://www.ncbi.nlm.nih.gov/pubmed/19223333
http://dx.doi.org/10.1093/nar/gkp065
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author Aparicio, Tomás
Guillou, Emmanuelle
Coloma, Javier
Montoya, Guillermo
Méndez, Juan
author_facet Aparicio, Tomás
Guillou, Emmanuelle
Coloma, Javier
Montoya, Guillermo
Méndez, Juan
author_sort Aparicio, Tomás
collection PubMed
description The GINS complex, originally discovered in Saccharomyces cerevisiae and Xenopus laevis, binds to DNA replication origins shortly before the onset of S phase and travels with the replication forks after initiation. In this study we present a detailed characterization of the human GINS (hGINS) homolog. Using new antibodies that allow the detection of endogenous hGINS in cells and tissues, we have examined its expression, abundance, subcellular localization and association with other DNA replication proteins. Expression of hGINS is restricted to actively proliferating cells. During the S phase, hGINS becomes part of a Cdc45–MCM–GINS (CMG) complex that is assembled on chromatin. Down-regulation of hGINS destabilizes CMG, causes a G1–S arrest and slows down ongoing DNA replication, effectively blocking cell proliferation. Our data support the notion that hGINS is an essential component of the human replisome.
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spelling pubmed-26734212009-05-15 The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication Aparicio, Tomás Guillou, Emmanuelle Coloma, Javier Montoya, Guillermo Méndez, Juan Nucleic Acids Res Genome Integrity, Repair and Replication The GINS complex, originally discovered in Saccharomyces cerevisiae and Xenopus laevis, binds to DNA replication origins shortly before the onset of S phase and travels with the replication forks after initiation. In this study we present a detailed characterization of the human GINS (hGINS) homolog. Using new antibodies that allow the detection of endogenous hGINS in cells and tissues, we have examined its expression, abundance, subcellular localization and association with other DNA replication proteins. Expression of hGINS is restricted to actively proliferating cells. During the S phase, hGINS becomes part of a Cdc45–MCM–GINS (CMG) complex that is assembled on chromatin. Down-regulation of hGINS destabilizes CMG, causes a G1–S arrest and slows down ongoing DNA replication, effectively blocking cell proliferation. Our data support the notion that hGINS is an essential component of the human replisome. Oxford University Press 2009-04 2009-02-17 /pmc/articles/PMC2673421/ /pubmed/19223333 http://dx.doi.org/10.1093/nar/gkp065 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Integrity, Repair and Replication
Aparicio, Tomás
Guillou, Emmanuelle
Coloma, Javier
Montoya, Guillermo
Méndez, Juan
The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication
title The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication
title_full The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication
title_fullStr The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication
title_full_unstemmed The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication
title_short The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication
title_sort human gins complex associates with cdc45 and mcm and is essential for dna replication
topic Genome Integrity, Repair and Replication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673421/
https://www.ncbi.nlm.nih.gov/pubmed/19223333
http://dx.doi.org/10.1093/nar/gkp065
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