DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision

No genes for any of the known uracil DNA glycosylases of the UDG superfamily are present in the genome of Methanothermobacter thermautotrophicus ΔH, making it difficult to imagine how DNA-U repair might be initiated in this organism. Recently, Mth212, the ExoIII homologue of M. thermautotrophicus ΔH...

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Autores principales: Schomacher, Lars, Chong, James P. J., McDermott, Paul, Kramer, Wilfried, Fritz, Hans-Joachim
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Genome Integrity, Repair and Replication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673441/
https://www.ncbi.nlm.nih.gov/pubmed/19240141
http://dx.doi.org/10.1093/nar/gkp102
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author Schomacher, Lars
Chong, James P. J.
McDermott, Paul
Kramer, Wilfried
Fritz, Hans-Joachim
author_facet Schomacher, Lars
Chong, James P. J.
McDermott, Paul
Kramer, Wilfried
Fritz, Hans-Joachim
author_sort Schomacher, Lars
collection PubMed
description No genes for any of the known uracil DNA glycosylases of the UDG superfamily are present in the genome of Methanothermobacter thermautotrophicus ΔH, making it difficult to imagine how DNA-U repair might be initiated in this organism. Recently, Mth212, the ExoIII homologue of M. thermautotrophicus ΔH has been characterized as a DNA uridine endonuclease, which suggested the possibility of a novel endonucleolytic entry mechanism for DNA uracil repair. With no system of genetic experimentation available, the problem was approached biochemically. Assays of DNA uracil repair in vitro, promoted by crude cellular extracts, provide unequivocal confirmation that this mechanism does indeed operate in M. thermautotrophicus ΔH.
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spelling pubmed-26734412009-05-15 DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision Schomacher, Lars Chong, James P. J. McDermott, Paul Kramer, Wilfried Fritz, Hans-Joachim Nucleic Acids Res Genome Integrity, Repair and Replication No genes for any of the known uracil DNA glycosylases of the UDG superfamily are present in the genome of Methanothermobacter thermautotrophicus ΔH, making it difficult to imagine how DNA-U repair might be initiated in this organism. Recently, Mth212, the ExoIII homologue of M. thermautotrophicus ΔH has been characterized as a DNA uridine endonuclease, which suggested the possibility of a novel endonucleolytic entry mechanism for DNA uracil repair. With no system of genetic experimentation available, the problem was approached biochemically. Assays of DNA uracil repair in vitro, promoted by crude cellular extracts, provide unequivocal confirmation that this mechanism does indeed operate in M. thermautotrophicus ΔH. Oxford University Press 2009-04 2009-02-24 /pmc/articles/PMC2673441/ /pubmed/19240141 http://dx.doi.org/10.1093/nar/gkp102 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Integrity, Repair and Replication
Schomacher, Lars
Chong, James P. J.
McDermott, Paul
Kramer, Wilfried
Fritz, Hans-Joachim
DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision
title DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision
title_full DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision
title_fullStr DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision
title_full_unstemmed DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision
title_short DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision
title_sort dna uracil repair initiated by the archaeal exoiii homologue mth212 via direct strand incision
topic Genome Integrity, Repair and Replication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673441/
https://www.ncbi.nlm.nih.gov/pubmed/19240141
http://dx.doi.org/10.1093/nar/gkp102
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