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Asymmetric DNA requirements in Xer recombination activation by FtsK

In bacteria with circular chromosomes, homologous recombination events can lead to the formation of chromosome dimers. In Escherichia coli, chromosome dimers are resolved by the addition of a crossover by two tyrosine recombinases, XerC and XerD, at a specific site on the chromosome, dif. Recombinat...

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Autores principales: Bonné, Laetitia, Bigot, Sarah, Chevalier, Fabien, Allemand, Jean-François, Barre, François-Xavier
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673442/
https://www.ncbi.nlm.nih.gov/pubmed/19246541
http://dx.doi.org/10.1093/nar/gkp104
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author Bonné, Laetitia
Bigot, Sarah
Chevalier, Fabien
Allemand, Jean-François
Barre, François-Xavier
author_facet Bonné, Laetitia
Bigot, Sarah
Chevalier, Fabien
Allemand, Jean-François
Barre, François-Xavier
author_sort Bonné, Laetitia
collection PubMed
description In bacteria with circular chromosomes, homologous recombination events can lead to the formation of chromosome dimers. In Escherichia coli, chromosome dimers are resolved by the addition of a crossover by two tyrosine recombinases, XerC and XerD, at a specific site on the chromosome, dif. Recombination depends on a direct contact between XerD and a cell division protein, FtsK, which functions as a hexameric double stranded DNA translocase. Here, we have investigated how the structure and composition of DNA interferes with Xer recombination activation by FtsK. XerC and XerD each cleave a specific strand on dif, the top and bottom strand, respectively. We found that the integrity and nature of eight bottom-strand nucleotides and three top-strand nucleotides immediately adjacent to the XerD-binding site of dif are crucial for recombination. These nucleotides are probably not implicated in FtsK translocation since FtsK could translocate on single stranded DNA in both the 5′–3′ and 3′–5′ orientation along a few nucleotides. We propose that they are required to stabilize FtsK in the vicinity of dif for recombination to occur because the FtsK–XerD interaction is too transient or too weak in itself to allow for XerD catalysis.
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spelling pubmed-26734422009-05-15 Asymmetric DNA requirements in Xer recombination activation by FtsK Bonné, Laetitia Bigot, Sarah Chevalier, Fabien Allemand, Jean-François Barre, François-Xavier Nucleic Acids Res Nucleic Acid Enzymes In bacteria with circular chromosomes, homologous recombination events can lead to the formation of chromosome dimers. In Escherichia coli, chromosome dimers are resolved by the addition of a crossover by two tyrosine recombinases, XerC and XerD, at a specific site on the chromosome, dif. Recombination depends on a direct contact between XerD and a cell division protein, FtsK, which functions as a hexameric double stranded DNA translocase. Here, we have investigated how the structure and composition of DNA interferes with Xer recombination activation by FtsK. XerC and XerD each cleave a specific strand on dif, the top and bottom strand, respectively. We found that the integrity and nature of eight bottom-strand nucleotides and three top-strand nucleotides immediately adjacent to the XerD-binding site of dif are crucial for recombination. These nucleotides are probably not implicated in FtsK translocation since FtsK could translocate on single stranded DNA in both the 5′–3′ and 3′–5′ orientation along a few nucleotides. We propose that they are required to stabilize FtsK in the vicinity of dif for recombination to occur because the FtsK–XerD interaction is too transient or too weak in itself to allow for XerD catalysis. Oxford University Press 2009-04 2009-02-26 /pmc/articles/PMC2673442/ /pubmed/19246541 http://dx.doi.org/10.1093/nar/gkp104 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nucleic Acid Enzymes
Bonné, Laetitia
Bigot, Sarah
Chevalier, Fabien
Allemand, Jean-François
Barre, François-Xavier
Asymmetric DNA requirements in Xer recombination activation by FtsK
title Asymmetric DNA requirements in Xer recombination activation by FtsK
title_full Asymmetric DNA requirements in Xer recombination activation by FtsK
title_fullStr Asymmetric DNA requirements in Xer recombination activation by FtsK
title_full_unstemmed Asymmetric DNA requirements in Xer recombination activation by FtsK
title_short Asymmetric DNA requirements in Xer recombination activation by FtsK
title_sort asymmetric dna requirements in xer recombination activation by ftsk
topic Nucleic Acid Enzymes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673442/
https://www.ncbi.nlm.nih.gov/pubmed/19246541
http://dx.doi.org/10.1093/nar/gkp104
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