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A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis

BACKGROUND: Ubiquitination is mediated by the sequential action of at least three enzymes: the E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme) and E3 (ubiquitin ligase) proteins. Polyubiquitination of target proteins is also implicated in several critical cellular processes. Alth...

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Detalles Bibliográficos
Autores principales:	Takahashi, Hirotaka, Nozawa, Akira, Seki, Motoaki, Shinozaki, Kazuo, Endo, Yaeta, Sawasaki, Tatsuya
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2674041/ https://www.ncbi.nlm.nih.gov/pubmed/19348673 http://dx.doi.org/10.1186/1471-2229-9-39

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