Structure of N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) from Mycobacterium tuberculosis in a cubic space group

GlmU is a bifunctional enzyme that catalyzes the final two steps in the biosynthesis of UDP-GlcNAc. Crystals of GlmU from Mycobacterium tuberculosis obtained using ammonium sulfate as a precipitant diffracted poorly (to 3.4 Å resolution) and displayed an unusually high solvent content (>80%) with...

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Autores principales: Verma, Sunil Kumar, Jaiswal, Mamta, Kumar, Neeraj, Parikh, Amit, Nandicoori, Vinay Kumar, Prakash, Balaji
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2009
Materias:
Structural Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2675579/
https://www.ncbi.nlm.nih.gov/pubmed/19407371
http://dx.doi.org/10.1107/S1744309109010252
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author Verma, Sunil Kumar
Jaiswal, Mamta
Kumar, Neeraj
Parikh, Amit
Nandicoori, Vinay Kumar
Prakash, Balaji
author_facet Verma, Sunil Kumar
Jaiswal, Mamta
Kumar, Neeraj
Parikh, Amit
Nandicoori, Vinay Kumar
Prakash, Balaji
author_sort Verma, Sunil Kumar
collection PubMed
description GlmU is a bifunctional enzyme that catalyzes the final two steps in the biosynthesis of UDP-GlcNAc. Crystals of GlmU from Mycobacterium tuberculosis obtained using ammonium sulfate as a precipitant diffracted poorly (to 3.4 Å resolution) and displayed an unusually high solvent content (>80%) with sparse crystal packing that resulted in large solvent channels. With one molecule per asymmetric unit, the monomers from three neighbouring asymmetric units related by the crystal threefold formed a biological trimer. Although this is the first report of the structure of GlmU determined in a cubic crystal form, the trimeric arrangement here is similar to that observed for other GlmU structures determined in hexagonal (H3, H32, P6(3)22) space groups.
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spelling pubmed-26755792009-05-06 Structure of N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) from Mycobacterium tuberculosis in a cubic space group Verma, Sunil Kumar Jaiswal, Mamta Kumar, Neeraj Parikh, Amit Nandicoori, Vinay Kumar Prakash, Balaji Acta Crystallogr Sect F Struct Biol Cryst Commun Structural Communications GlmU is a bifunctional enzyme that catalyzes the final two steps in the biosynthesis of UDP-GlcNAc. Crystals of GlmU from Mycobacterium tuberculosis obtained using ammonium sulfate as a precipitant diffracted poorly (to 3.4 Å resolution) and displayed an unusually high solvent content (>80%) with sparse crystal packing that resulted in large solvent channels. With one molecule per asymmetric unit, the monomers from three neighbouring asymmetric units related by the crystal threefold formed a biological trimer. Although this is the first report of the structure of GlmU determined in a cubic crystal form, the trimeric arrangement here is similar to that observed for other GlmU structures determined in hexagonal (H3, H32, P6(3)22) space groups. International Union of Crystallography 2009-04-24 /pmc/articles/PMC2675579/ /pubmed/19407371 http://dx.doi.org/10.1107/S1744309109010252 Text en © Verma et al. 2009 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Structural Communications
Verma, Sunil Kumar
Jaiswal, Mamta
Kumar, Neeraj
Parikh, Amit
Nandicoori, Vinay Kumar
Prakash, Balaji
Structure of N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) from Mycobacterium tuberculosis in a cubic space group
title Structure of N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) from Mycobacterium tuberculosis in a cubic space group
title_full Structure of N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) from Mycobacterium tuberculosis in a cubic space group
title_fullStr Structure of N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) from Mycobacterium tuberculosis in a cubic space group
title_full_unstemmed Structure of N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) from Mycobacterium tuberculosis in a cubic space group
title_short Structure of N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) from Mycobacterium tuberculosis in a cubic space group
title_sort structure of n-acetylglucosamine-1-phosphate uridyltransferase (glmu) from mycobacterium tuberculosis in a cubic space group
topic Structural Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2675579/
https://www.ncbi.nlm.nih.gov/pubmed/19407371
http://dx.doi.org/10.1107/S1744309109010252
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