Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9

Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cl...

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Detalles Bibliográficos
Autores principales: Wang, Wei, Wei, Lei, Yang, Anqi, He, Teng, Yuen, K. Y., Chen, Cheng, Rao, Zihe
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2009
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2675602/
https://www.ncbi.nlm.nih.gov/pubmed/19407394
http://dx.doi.org/10.1107/S1744309109014055
Descripción
Sumario:Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 Å resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 Å, α = β = γ = 90° and two molecules per asymmetric unit.

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