Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9

Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cl...

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Autores principales: Wang, Wei, Wei, Lei, Yang, Anqi, He, Teng, Yuen, K. Y., Chen, Cheng, Rao, Zihe
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2009
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2675602/
https://www.ncbi.nlm.nih.gov/pubmed/19407394
http://dx.doi.org/10.1107/S1744309109014055
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author Wang, Wei
Wei, Lei
Yang, Anqi
He, Teng
Yuen, K. Y.
Chen, Cheng
Rao, Zihe
author_facet Wang, Wei
Wei, Lei
Yang, Anqi
He, Teng
Yuen, K. Y.
Chen, Cheng
Rao, Zihe
author_sort Wang, Wei
collection PubMed
description Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 Å resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 Å, α = β = γ = 90° and two molecules per asymmetric unit.
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spelling pubmed-26756022011-05-01 Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9 Wang, Wei Wei, Lei Yang, Anqi He, Teng Yuen, K. Y. Chen, Cheng Rao, Zihe Acta Crystallogr Sect F Struct Biol Cryst Commun Crystallization Communications Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 Å resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 Å, α = β = γ = 90° and two molecules per asymmetric unit. International Union of Crystallography 2009-04-28 /pmc/articles/PMC2675602/ /pubmed/19407394 http://dx.doi.org/10.1107/S1744309109014055 Text en © International Union of Crystallography 2009
spellingShingle Crystallization Communications
Wang, Wei
Wei, Lei
Yang, Anqi
He, Teng
Yuen, K. Y.
Chen, Cheng
Rao, Zihe
Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
title Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
title_full Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
title_fullStr Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
title_full_unstemmed Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
title_short Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
title_sort expression, crystallization and preliminary crystallographic study of human coronavirus hku1 nonstructural protein 9
topic Crystallization Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2675602/
https://www.ncbi.nlm.nih.gov/pubmed/19407394
http://dx.doi.org/10.1107/S1744309109014055
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