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Proinflammatory cytokines in a mouse model of central retinal artery occlusion

PURPOSE: To analyze cytokines in the retina and serum in an experimental model of central retinal artery occlusion (CRAO) in mice. METHODS: CRAO was induced by laser activation of intravenously injected rose bengal, a photosensitive dye, in 60 C57Bl/6 mice. mRNA and protein levels of macrophage inhi...

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Autores principales: Kramer, Michal, Dadon, S., Hasanreisoglu, M., Monselise, Y., Avraham, B. R., Feldman, A., Eldar, I., Weinberger, D., Goldenberg-Cohen, N.
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2676200/
https://www.ncbi.nlm.nih.gov/pubmed/19421412
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author Kramer, Michal
Dadon, S.
Hasanreisoglu, M.
Monselise, Y.
Avraham, B. R.
Feldman, A.
Eldar, I.
Weinberger, D.
Goldenberg-Cohen, N.
author_facet Kramer, Michal
Dadon, S.
Hasanreisoglu, M.
Monselise, Y.
Avraham, B. R.
Feldman, A.
Eldar, I.
Weinberger, D.
Goldenberg-Cohen, N.
author_sort Kramer, Michal
collection PubMed
description PURPOSE: To analyze cytokines in the retina and serum in an experimental model of central retinal artery occlusion (CRAO) in mice. METHODS: CRAO was induced by laser activation of intravenously injected rose bengal, a photosensitive dye, in 60 C57Bl/6 mice. mRNA and protein levels of macrophage inhibitory protein-2 (MIP-2), interleukin-6 (IL-6), and tumor necrosis factor- α (TNF-α) were analyzed using real-time polymerase chain reaction, and western blot, respectively. Cytokine levels in serum were measured by ELISA. Analysis was performed at various time intervals from CRAO induction. RESULTS: In the retina, MIP-2 and IL-6 mRNA expression decreased 3 h after induction of CRAO and increased thereafter, peaking at 12–24 h. By 7 days, levels were again mostly undetectable. TNF-α mRNA expression increased at 3 h and decreased to control levels at 7 days. At the protein level, all cytokines were present at 3 h, following similar patterns to their respective gene expression thereafter. In serum, MIP-2 and TNF-α levels peaked early, and decreased to control levels at 12 h, with a second late rise of TNF-α. IL-6 levels increased between 3 and 12 h and decreased at 24 h. CONCLUSIONS: Temporal variations in cytokines were observed following the induction of CRAO, both at the retinal mRNA expression and protein levels. These temporal changes, and the variable effects of the cytokines at the different time intervals, should be taken into account during the formulation of therapeutic strategies.
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spelling pubmed-26762002009-05-05 Proinflammatory cytokines in a mouse model of central retinal artery occlusion Kramer, Michal Dadon, S. Hasanreisoglu, M. Monselise, Y. Avraham, B. R. Feldman, A. Eldar, I. Weinberger, D. Goldenberg-Cohen, N. Mol Vis Research Article PURPOSE: To analyze cytokines in the retina and serum in an experimental model of central retinal artery occlusion (CRAO) in mice. METHODS: CRAO was induced by laser activation of intravenously injected rose bengal, a photosensitive dye, in 60 C57Bl/6 mice. mRNA and protein levels of macrophage inhibitory protein-2 (MIP-2), interleukin-6 (IL-6), and tumor necrosis factor- α (TNF-α) were analyzed using real-time polymerase chain reaction, and western blot, respectively. Cytokine levels in serum were measured by ELISA. Analysis was performed at various time intervals from CRAO induction. RESULTS: In the retina, MIP-2 and IL-6 mRNA expression decreased 3 h after induction of CRAO and increased thereafter, peaking at 12–24 h. By 7 days, levels were again mostly undetectable. TNF-α mRNA expression increased at 3 h and decreased to control levels at 7 days. At the protein level, all cytokines were present at 3 h, following similar patterns to their respective gene expression thereafter. In serum, MIP-2 and TNF-α levels peaked early, and decreased to control levels at 12 h, with a second late rise of TNF-α. IL-6 levels increased between 3 and 12 h and decreased at 24 h. CONCLUSIONS: Temporal variations in cytokines were observed following the induction of CRAO, both at the retinal mRNA expression and protein levels. These temporal changes, and the variable effects of the cytokines at the different time intervals, should be taken into account during the formulation of therapeutic strategies. Molecular Vision 2009-05-01 /pmc/articles/PMC2676200/ /pubmed/19421412 Text en http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kramer, Michal
Dadon, S.
Hasanreisoglu, M.
Monselise, Y.
Avraham, B. R.
Feldman, A.
Eldar, I.
Weinberger, D.
Goldenberg-Cohen, N.
Proinflammatory cytokines in a mouse model of central retinal artery occlusion
title Proinflammatory cytokines in a mouse model of central retinal artery occlusion
title_full Proinflammatory cytokines in a mouse model of central retinal artery occlusion
title_fullStr Proinflammatory cytokines in a mouse model of central retinal artery occlusion
title_full_unstemmed Proinflammatory cytokines in a mouse model of central retinal artery occlusion
title_short Proinflammatory cytokines in a mouse model of central retinal artery occlusion
title_sort proinflammatory cytokines in a mouse model of central retinal artery occlusion
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2676200/
https://www.ncbi.nlm.nih.gov/pubmed/19421412
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