Zinc tetraaminophthalocyanine-Fe(3)O(4) nanoparticle composite for laccase immobilization

Zinc tetraaminophthalocyanine-Fe(3)O(4) nanoparticle composites were prepared by organic-inorganic complex technology and characterized. It has been proved that the ZnTAPc dispersed randomly onto the surface of Fe(3)O(4) nanoparticles to form molecular dispersion layer and there was a relatively strong bond between central zinc cation and oxygen. The nanoparticle composite took the shape of roundish spheres with the mean diameter of about 15 nm. Active amino groups of magnetic carriers could be used to bind laccase via glutaraldehyde. The optimal pH for the activity of the immobilized laccases and free laccase were the same at pH 3.0 and the optimal temperature for laccase immobilization on ZnTAPc-Fe(3)O(4) nanoparticle composite was 45°. The immobilization yields and K(m) value of the laccase immobilized on ZnTAPc-Fe(3)O(4) nanoparticle composite were 25% and 20.1 μM, respectively. This kind of immobilized laccase has good thermal, storage and operation stability, and could be used as the sensing biocomponent for the fiber optic biosensor based on enzyme catalysis.