Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds

DNA strands coated with AuNPs were cleaved by restriction enzymes while in solution or on a surface. Enzymatic activity was verified by gel electrophoresis prior to surface analysis. Cleavage results suggest that enzymes can recognize the AuNP-coated strands while on the surfaces, though specificity...

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Detalles Bibliográficos
Autores principales: Slavin, John WJ, Ivanisevic, Albena
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2007
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2676810/
https://www.ncbi.nlm.nih.gov/pubmed/18203449
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author Slavin, John WJ
Ivanisevic, Albena
author_facet Slavin, John WJ
Ivanisevic, Albena
author_sort Slavin, John WJ
collection PubMed
description DNA strands coated with AuNPs were cleaved by restriction enzymes while in solution or on a surface. Enzymatic activity was verified by gel electrophoresis prior to surface analysis. Cleavage results suggest that enzymes can recognize the AuNP-coated strands while on the surfaces, though specificity in digestion has not yet been verified. Development allows for advances in site specific localization of components using biological media.
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spelling pubmed-26768102009-05-12 Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds Slavin, John WJ Ivanisevic, Albena Int J Nanomedicine Original Research DNA strands coated with AuNPs were cleaved by restriction enzymes while in solution or on a surface. Enzymatic activity was verified by gel electrophoresis prior to surface analysis. Cleavage results suggest that enzymes can recognize the AuNP-coated strands while on the surfaces, though specificity in digestion has not yet been verified. Development allows for advances in site specific localization of components using biological media. Dove Medical Press 2007-12 2007-12 /pmc/articles/PMC2676810/ /pubmed/18203449 Text en © 2007 Dove Medical Press Limited. All rights reserved
spellingShingle Original Research
Slavin, John WJ
Ivanisevic, Albena
Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds
title Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds
title_full Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds
title_fullStr Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds
title_full_unstemmed Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds
title_short Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds
title_sort dual restriction enzyme digest of cationic-gold-coated dna scaffolds
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2676810/
https://www.ncbi.nlm.nih.gov/pubmed/18203449
work_keys_str_mv AT slavinjohnwj dualrestrictionenzymedigestofcationicgoldcoateddnascaffolds
AT ivanisevicalbena dualrestrictionenzymedigestofcationicgoldcoateddnascaffolds

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