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Primitive templated catalysis of a peptide ligation by self-folding RNAs
RNA–polypeptide complexes (RNPs), which play various roles in extant biological systems, have been suggested to have been important in the early stages of the molecular evolution of life. At a certain developmental stage of ancient RNPs, their RNA and polypeptide components have been proposed to evo...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677871/ https://www.ncbi.nlm.nih.gov/pubmed/19264804 http://dx.doi.org/10.1093/nar/gkp111 |
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author | Kashiwagi, Norimasa Furuta, Hiroyuki Ikawa, Yoshiya |
author_facet | Kashiwagi, Norimasa Furuta, Hiroyuki Ikawa, Yoshiya |
author_sort | Kashiwagi, Norimasa |
collection | PubMed |
description | RNA–polypeptide complexes (RNPs), which play various roles in extant biological systems, have been suggested to have been important in the early stages of the molecular evolution of life. At a certain developmental stage of ancient RNPs, their RNA and polypeptide components have been proposed to evolve in a reciprocal manner to establish highly elaborate structures and functions. We have constructed a simple model system, from which a cooperative evolution system of RNA and polypeptide components could be developed. Based on the observation that several RNAs modestly accelerated the chemical ligation of the two basic peptides. We have designed an RNA molecule possessing two peptide binding sites that capture the two peptides. This designed RNA can also accelerate the peptide ligation. The resulting ligated peptide, which has two RNA-binding sites, can in turn function as a trans-acting factor that enhances the endonuclease activity catalyzed by the designed RNA. |
format | Text |
id | pubmed-2677871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-26778712009-05-15 Primitive templated catalysis of a peptide ligation by self-folding RNAs Kashiwagi, Norimasa Furuta, Hiroyuki Ikawa, Yoshiya Nucleic Acids Res RNA RNA–polypeptide complexes (RNPs), which play various roles in extant biological systems, have been suggested to have been important in the early stages of the molecular evolution of life. At a certain developmental stage of ancient RNPs, their RNA and polypeptide components have been proposed to evolve in a reciprocal manner to establish highly elaborate structures and functions. We have constructed a simple model system, from which a cooperative evolution system of RNA and polypeptide components could be developed. Based on the observation that several RNAs modestly accelerated the chemical ligation of the two basic peptides. We have designed an RNA molecule possessing two peptide binding sites that capture the two peptides. This designed RNA can also accelerate the peptide ligation. The resulting ligated peptide, which has two RNA-binding sites, can in turn function as a trans-acting factor that enhances the endonuclease activity catalyzed by the designed RNA. Oxford University Press 2009-05 2009-03-05 /pmc/articles/PMC2677871/ /pubmed/19264804 http://dx.doi.org/10.1093/nar/gkp111 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA Kashiwagi, Norimasa Furuta, Hiroyuki Ikawa, Yoshiya Primitive templated catalysis of a peptide ligation by self-folding RNAs |
title | Primitive templated catalysis of a peptide ligation by self-folding RNAs |
title_full | Primitive templated catalysis of a peptide ligation by self-folding RNAs |
title_fullStr | Primitive templated catalysis of a peptide ligation by self-folding RNAs |
title_full_unstemmed | Primitive templated catalysis of a peptide ligation by self-folding RNAs |
title_short | Primitive templated catalysis of a peptide ligation by self-folding RNAs |
title_sort | primitive templated catalysis of a peptide ligation by self-folding rnas |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2677871/ https://www.ncbi.nlm.nih.gov/pubmed/19264804 http://dx.doi.org/10.1093/nar/gkp111 |
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