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Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantation
We studied serum proteomic profiling in patients with graft versus host disease (GVHD) after allogeneic hematopoietic cell transplantation (allo-HCT) by two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. The expression of a group of proteins, haptoglobin (Hp), alpha-1-antitry...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2678154/ https://www.ncbi.nlm.nih.gov/pubmed/19379511 http://dx.doi.org/10.1186/1756-8722-2-17 |
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author | McGuirk, Joseph Hao, Gang Hou, Weijian Abhyankar, Sunil Williams, Casey Yan, Weisi Yuan, Jianda Guan, Xiuqin Belt, Robert Dejarnette, Shaun Wieman, Jeffery Yan, Ying |
author_facet | McGuirk, Joseph Hao, Gang Hou, Weijian Abhyankar, Sunil Williams, Casey Yan, Weisi Yuan, Jianda Guan, Xiuqin Belt, Robert Dejarnette, Shaun Wieman, Jeffery Yan, Ying |
author_sort | McGuirk, Joseph |
collection | PubMed |
description | We studied serum proteomic profiling in patients with graft versus host disease (GVHD) after allogeneic hematopoietic cell transplantation (allo-HCT) by two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. The expression of a group of proteins, haptoglobin (Hp), alpha-1-antitrypsin, apolipoprotein A-IV, serum paraoxonase and Zn-alpha-glycoprotein were increased and the proteins, clusterin precursor, alpha-2-macroglobulin, serum amyloid protein precursor, sex hormone-binding globulin, serotransferrin and complement C4 were decreased in patients with extensive chronic GVHD (cGVHD). Serum haptoglobin (Hp) levels in patients with cGVHD were demonstrated to be statistically higher than in patients without cGVHD and normal controls (p < 0.01). We used immunoblotting and PCR in combination with 2-DE gel image analysis to determine Hp polymorphisms in 25 allo-HCT patients and 16 normal donors. The results demonstrate that patients with cGVHD had a higher incidence of HP 2-2 phenotype (43.8%), in comparison to the patients without cGVHD (0%) and normal donors (18.7%), suggesting the possibility that specific Hp polymorphism may play a role in the development of cGVHD after allo-HCT. In this study, quantitative serum Hp levels were shown to be related to cGVHD development. Further, the data suggest the possibility that specific Hp polymorphisms may be associated with cGVHD development and warrant further investigation. |
format | Text |
id | pubmed-2678154 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26781542009-05-07 Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantation McGuirk, Joseph Hao, Gang Hou, Weijian Abhyankar, Sunil Williams, Casey Yan, Weisi Yuan, Jianda Guan, Xiuqin Belt, Robert Dejarnette, Shaun Wieman, Jeffery Yan, Ying J Hematol Oncol Research We studied serum proteomic profiling in patients with graft versus host disease (GVHD) after allogeneic hematopoietic cell transplantation (allo-HCT) by two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. The expression of a group of proteins, haptoglobin (Hp), alpha-1-antitrypsin, apolipoprotein A-IV, serum paraoxonase and Zn-alpha-glycoprotein were increased and the proteins, clusterin precursor, alpha-2-macroglobulin, serum amyloid protein precursor, sex hormone-binding globulin, serotransferrin and complement C4 were decreased in patients with extensive chronic GVHD (cGVHD). Serum haptoglobin (Hp) levels in patients with cGVHD were demonstrated to be statistically higher than in patients without cGVHD and normal controls (p < 0.01). We used immunoblotting and PCR in combination with 2-DE gel image analysis to determine Hp polymorphisms in 25 allo-HCT patients and 16 normal donors. The results demonstrate that patients with cGVHD had a higher incidence of HP 2-2 phenotype (43.8%), in comparison to the patients without cGVHD (0%) and normal donors (18.7%), suggesting the possibility that specific Hp polymorphism may play a role in the development of cGVHD after allo-HCT. In this study, quantitative serum Hp levels were shown to be related to cGVHD development. Further, the data suggest the possibility that specific Hp polymorphisms may be associated with cGVHD development and warrant further investigation. BioMed Central 2009-04-20 /pmc/articles/PMC2678154/ /pubmed/19379511 http://dx.doi.org/10.1186/1756-8722-2-17 Text en Copyright © 2009 McGuirk et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research McGuirk, Joseph Hao, Gang Hou, Weijian Abhyankar, Sunil Williams, Casey Yan, Weisi Yuan, Jianda Guan, Xiuqin Belt, Robert Dejarnette, Shaun Wieman, Jeffery Yan, Ying Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantation |
title | Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantation |
title_full | Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantation |
title_fullStr | Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantation |
title_full_unstemmed | Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantation |
title_short | Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantation |
title_sort | serum proteomic profiling and haptoglobin polymorphisms in patients with gvhd after allogeneic hematopoietic cell transplantation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2678154/ https://www.ncbi.nlm.nih.gov/pubmed/19379511 http://dx.doi.org/10.1186/1756-8722-2-17 |
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