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The complete genome sequence of Staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic Crenarchaeota

BACKGROUND: Staphylothermus marinus is an anaerobic, sulfur-reducing peptide fermenter of the archaeal phylum Crenarchaeota. It is the third heterotrophic, obligate sulfur reducing crenarchaeote to be sequenced and provides an opportunity for comparative analysis of the three genomes. RESULTS: The 1...

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Autores principales: Anderson, Iain J, Dharmarajan, Lakshmi, Rodriguez, Jason, Hooper, Sean, Porat, Iris, Ulrich, Luke E, Elkins, James G, Mavromatis, Kostas, Sun, Hui, Land, Miriam, Lapidus, Alla, Lucas, Susan, Barry, Kerrie, Huber, Harald, Zhulin, Igor B, Whitman, William B, Mukhopadhyay, Biswarup, Woese, Carl, Bristow, James, Kyrpides, Nikos
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2678158/
https://www.ncbi.nlm.nih.gov/pubmed/19341479
http://dx.doi.org/10.1186/1471-2164-10-145
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author Anderson, Iain J
Dharmarajan, Lakshmi
Rodriguez, Jason
Hooper, Sean
Porat, Iris
Ulrich, Luke E
Elkins, James G
Mavromatis, Kostas
Sun, Hui
Land, Miriam
Lapidus, Alla
Lucas, Susan
Barry, Kerrie
Huber, Harald
Zhulin, Igor B
Whitman, William B
Mukhopadhyay, Biswarup
Woese, Carl
Bristow, James
Kyrpides, Nikos
author_facet Anderson, Iain J
Dharmarajan, Lakshmi
Rodriguez, Jason
Hooper, Sean
Porat, Iris
Ulrich, Luke E
Elkins, James G
Mavromatis, Kostas
Sun, Hui
Land, Miriam
Lapidus, Alla
Lucas, Susan
Barry, Kerrie
Huber, Harald
Zhulin, Igor B
Whitman, William B
Mukhopadhyay, Biswarup
Woese, Carl
Bristow, James
Kyrpides, Nikos
author_sort Anderson, Iain J
collection PubMed
description BACKGROUND: Staphylothermus marinus is an anaerobic, sulfur-reducing peptide fermenter of the archaeal phylum Crenarchaeota. It is the third heterotrophic, obligate sulfur reducing crenarchaeote to be sequenced and provides an opportunity for comparative analysis of the three genomes. RESULTS: The 1.57 Mbp genome of the hyperthermophilic crenarchaeote Staphylothermus marinus has been completely sequenced. The main energy generating pathways likely involve 2-oxoacid:ferredoxin oxidoreductases and ADP-forming acetyl-CoA synthases. S. marinus possesses several enzymes not present in other crenarchaeotes including a sodium ion-translocating decarboxylase likely to be involved in amino acid degradation. S. marinus lacks sulfur-reducing enzymes present in the other two sulfur-reducing crenarchaeotes that have been sequenced – Thermofilum pendens and Hyperthermus butylicus. Instead it has three operons similar to the mbh and mbx operons of Pyrococcus furiosus, which may play a role in sulfur reduction and/or hydrogen production. The two marine organisms, S. marinus and H. butylicus, possess more sodium-dependent transporters than T. pendens and use symporters for potassium uptake while T. pendens uses an ATP-dependent potassium transporter. T. pendens has adapted to a nutrient-rich environment while H. butylicus is adapted to a nutrient-poor environment, and S. marinus lies between these two extremes. CONCLUSION: The three heterotrophic sulfur-reducing crenarchaeotes have adapted to their habitats, terrestrial vs. marine, via their transporter content, and they have also adapted to environments with differing levels of nutrients. Despite the fact that they all use sulfur as an electron acceptor, they are likely to have different pathways for sulfur reduction.
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spelling pubmed-26781582009-05-07 The complete genome sequence of Staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic Crenarchaeota Anderson, Iain J Dharmarajan, Lakshmi Rodriguez, Jason Hooper, Sean Porat, Iris Ulrich, Luke E Elkins, James G Mavromatis, Kostas Sun, Hui Land, Miriam Lapidus, Alla Lucas, Susan Barry, Kerrie Huber, Harald Zhulin, Igor B Whitman, William B Mukhopadhyay, Biswarup Woese, Carl Bristow, James Kyrpides, Nikos BMC Genomics Research Article BACKGROUND: Staphylothermus marinus is an anaerobic, sulfur-reducing peptide fermenter of the archaeal phylum Crenarchaeota. It is the third heterotrophic, obligate sulfur reducing crenarchaeote to be sequenced and provides an opportunity for comparative analysis of the three genomes. RESULTS: The 1.57 Mbp genome of the hyperthermophilic crenarchaeote Staphylothermus marinus has been completely sequenced. The main energy generating pathways likely involve 2-oxoacid:ferredoxin oxidoreductases and ADP-forming acetyl-CoA synthases. S. marinus possesses several enzymes not present in other crenarchaeotes including a sodium ion-translocating decarboxylase likely to be involved in amino acid degradation. S. marinus lacks sulfur-reducing enzymes present in the other two sulfur-reducing crenarchaeotes that have been sequenced – Thermofilum pendens and Hyperthermus butylicus. Instead it has three operons similar to the mbh and mbx operons of Pyrococcus furiosus, which may play a role in sulfur reduction and/or hydrogen production. The two marine organisms, S. marinus and H. butylicus, possess more sodium-dependent transporters than T. pendens and use symporters for potassium uptake while T. pendens uses an ATP-dependent potassium transporter. T. pendens has adapted to a nutrient-rich environment while H. butylicus is adapted to a nutrient-poor environment, and S. marinus lies between these two extremes. CONCLUSION: The three heterotrophic sulfur-reducing crenarchaeotes have adapted to their habitats, terrestrial vs. marine, via their transporter content, and they have also adapted to environments with differing levels of nutrients. Despite the fact that they all use sulfur as an electron acceptor, they are likely to have different pathways for sulfur reduction. BioMed Central 2009-04-02 /pmc/articles/PMC2678158/ /pubmed/19341479 http://dx.doi.org/10.1186/1471-2164-10-145 Text en Copyright © 2009 Anderson et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Anderson, Iain J
Dharmarajan, Lakshmi
Rodriguez, Jason
Hooper, Sean
Porat, Iris
Ulrich, Luke E
Elkins, James G
Mavromatis, Kostas
Sun, Hui
Land, Miriam
Lapidus, Alla
Lucas, Susan
Barry, Kerrie
Huber, Harald
Zhulin, Igor B
Whitman, William B
Mukhopadhyay, Biswarup
Woese, Carl
Bristow, James
Kyrpides, Nikos
The complete genome sequence of Staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic Crenarchaeota
title The complete genome sequence of Staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic Crenarchaeota
title_full The complete genome sequence of Staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic Crenarchaeota
title_fullStr The complete genome sequence of Staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic Crenarchaeota
title_full_unstemmed The complete genome sequence of Staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic Crenarchaeota
title_short The complete genome sequence of Staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic Crenarchaeota
title_sort complete genome sequence of staphylothermus marinus reveals differences in sulfur metabolism among heterotrophic crenarchaeota
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2678158/
https://www.ncbi.nlm.nih.gov/pubmed/19341479
http://dx.doi.org/10.1186/1471-2164-10-145
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