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Reverse transcription-quantitative polymerase chain reaction: description of a RIN-based algorithm for accurate data normalization
BACKGROUND: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard technique for mRNA quantification, but appropriate normalization is required to obtain reliable data. Normalization to accurately quantitated RNA has been proposed as the most reliable method for...
Autores principales: | Ho-Pun-Cheung, Alexandre, Bascoul-Mollevi, Caroline, Assenat, Eric, Boissière-Michot, Florence, Bibeau, Frédéric, Cellier, Dominic, Ychou, Marc, Lopez-Crapez, Evelyne |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2679744/ https://www.ncbi.nlm.nih.gov/pubmed/19368728 http://dx.doi.org/10.1186/1471-2199-10-31 |
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