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Snail1 Protein in the Stroma as a New Putative Prognosis Marker for Colon Tumours
Over-expression of Snail1 gene transcriptional repressor promotes an epithelial-to-mesenchymal transition in epithelial tumour cell lines. Expression of Snail1 RNA has been associated to the pathogenesis of a number of malignancies; however, the lack of good monoclonal antibodies against this protei...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680015/ https://www.ncbi.nlm.nih.gov/pubmed/19440385 http://dx.doi.org/10.1371/journal.pone.0005595 |
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author | Francí, Clara Gallén, Manel Alameda, Francesc Baró, Teresa Iglesias, Mar Virtanen, Ismo García de Herreros, Antonio |
author_facet | Francí, Clara Gallén, Manel Alameda, Francesc Baró, Teresa Iglesias, Mar Virtanen, Ismo García de Herreros, Antonio |
author_sort | Francí, Clara |
collection | PubMed |
description | Over-expression of Snail1 gene transcriptional repressor promotes an epithelial-to-mesenchymal transition in epithelial tumour cell lines. Expression of Snail1 RNA has been associated to the pathogenesis of a number of malignancies; however, the lack of good monoclonal antibodies against this protein has precluded a definitive analysis of Snail1 protein. In this study, we aimed to determine the expression of this transcriptional factor in colorectal tumours. Using a Snail1 well-characterized monoclonal antibody developed in our laboratories we have analyzed by immunohistochemistry a cohort of 162 human colorectal tumours. Ninety tumours (56%) showed nuclear expression in the tumoral tissue and the adjacent stroma; in 34 (21%), Snail1 was detected just in the stroma, whereas in only 4 the expression of Snail1 was detected in the tumoral tissue and the stroma was negative. No correlation was found between the presence of Snail1 in the tumour and tumour stage; however, a trend (p = 0.054) was detected when the expression of this factor in the stroma was considered. Snail1 immunoreactivity in this compartment was associated with presence of distant metastasis (p = 0.006). Moreover, expression of Snail1 in the tumor stroma correlated with lower specific survival of cancer patients (p = 0.011). Interestingly, this correlation was also detected in stage I and II tumors. Therefore, our results indicate that the presence of nuclear Snail1 immunoreactive cells in the stroma may be an informative indicator of prognosis of colon tumours especially useful in those corresponding to lower stages and identify a new marker suitable to label activated stroma in colon tumours. |
format | Text |
id | pubmed-2680015 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26800152009-05-18 Snail1 Protein in the Stroma as a New Putative Prognosis Marker for Colon Tumours Francí, Clara Gallén, Manel Alameda, Francesc Baró, Teresa Iglesias, Mar Virtanen, Ismo García de Herreros, Antonio PLoS One Research Article Over-expression of Snail1 gene transcriptional repressor promotes an epithelial-to-mesenchymal transition in epithelial tumour cell lines. Expression of Snail1 RNA has been associated to the pathogenesis of a number of malignancies; however, the lack of good monoclonal antibodies against this protein has precluded a definitive analysis of Snail1 protein. In this study, we aimed to determine the expression of this transcriptional factor in colorectal tumours. Using a Snail1 well-characterized monoclonal antibody developed in our laboratories we have analyzed by immunohistochemistry a cohort of 162 human colorectal tumours. Ninety tumours (56%) showed nuclear expression in the tumoral tissue and the adjacent stroma; in 34 (21%), Snail1 was detected just in the stroma, whereas in only 4 the expression of Snail1 was detected in the tumoral tissue and the stroma was negative. No correlation was found between the presence of Snail1 in the tumour and tumour stage; however, a trend (p = 0.054) was detected when the expression of this factor in the stroma was considered. Snail1 immunoreactivity in this compartment was associated with presence of distant metastasis (p = 0.006). Moreover, expression of Snail1 in the tumor stroma correlated with lower specific survival of cancer patients (p = 0.011). Interestingly, this correlation was also detected in stage I and II tumors. Therefore, our results indicate that the presence of nuclear Snail1 immunoreactive cells in the stroma may be an informative indicator of prognosis of colon tumours especially useful in those corresponding to lower stages and identify a new marker suitable to label activated stroma in colon tumours. Public Library of Science 2009-05-18 /pmc/articles/PMC2680015/ /pubmed/19440385 http://dx.doi.org/10.1371/journal.pone.0005595 Text en Francí et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Francí, Clara Gallén, Manel Alameda, Francesc Baró, Teresa Iglesias, Mar Virtanen, Ismo García de Herreros, Antonio Snail1 Protein in the Stroma as a New Putative Prognosis Marker for Colon Tumours |
title | Snail1 Protein in the Stroma as a New Putative Prognosis Marker for Colon Tumours |
title_full | Snail1 Protein in the Stroma as a New Putative Prognosis Marker for Colon Tumours |
title_fullStr | Snail1 Protein in the Stroma as a New Putative Prognosis Marker for Colon Tumours |
title_full_unstemmed | Snail1 Protein in the Stroma as a New Putative Prognosis Marker for Colon Tumours |
title_short | Snail1 Protein in the Stroma as a New Putative Prognosis Marker for Colon Tumours |
title_sort | snail1 protein in the stroma as a new putative prognosis marker for colon tumours |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680015/ https://www.ncbi.nlm.nih.gov/pubmed/19440385 http://dx.doi.org/10.1371/journal.pone.0005595 |
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