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Germinal centre and marginal zone B cells expand quickly in a second Plasmodium chabaudi malaria infection producing mature plasma cells

Antibodies and B cells are critical in the protective immune response to the blood stage of the malaria parasite, Plasmodium chabaudi. However, little is known about the development of memory B cells and their differentiation into plasma cells during infection or after re-infection. Here we have sho...

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Detalles Bibliográficos
Autores principales: STEPHENS, R, NDUNGU, F M, LANGHORNE, J
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680269/
https://www.ncbi.nlm.nih.gov/pubmed/19121080
http://dx.doi.org/10.1111/j.1365-3024.2008.01066.x
Descripción
Sumario:Antibodies and B cells are critical in the protective immune response to the blood stage of the malaria parasite, Plasmodium chabaudi. However, little is known about the development of memory B cells and their differentiation into plasma cells during infection or after re-infection. Here we have shown that B cells with phenotypic characteristics of memory cells (CD19(+)IgD(−) CD38(+), IgG1(+)) are generated in a primaryPlasmodium chabaudi chabaudi infection of mice. In addition, we observed that germinal centre cells (CD19(+), GL7(+), MHCII(hi)) and Marginal Zone B cells (CD19(+)CD23(−)IgD(−)) show faster expansion on re-infection than in the primary, though other subsets do not. Interestingly, though both IgM(−) and IgM(+) memory cells are produced, IgM(+) memory cells do not expand on second infection. The second infection quickly produced mature bone marrow plasma cells (intracellular Ig(hi), CD138(hi), CD9(+), B220(−)), compared to primary infection; which generates a very large population of immature splenic plasma cells (B220+). This analysis suggests that a memory B cell population is generated after a single infection of malaria, which on re-infection responds quickly producing germinal centres and generating long-lived plasma cells making the second encounter with parasite more efficient.