Cargando…

Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels

BACKGROUND: Low birth weight has been related to an increased risk for developing high blood pressure in adult life. The molecular and cellular analysis of umbilical cord artery and vein may provide information about the early vascular characteristics of an individual. We have assessed several pheno...

Descripción completa

Detalles Bibliográficos
Autores principales: Martín de Llano, José Javier, Fuertes, Graciela, Torró, Isabel, García Vicent, Consuelo, Fayos, José Luis, Lurbe, Empar
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680391/
https://www.ncbi.nlm.nih.gov/pubmed/19393064
http://dx.doi.org/10.1186/1479-5876-7-30
_version_ 1782166947887054848
author Martín de Llano, José Javier
Fuertes, Graciela
Torró, Isabel
García Vicent, Consuelo
Fayos, José Luis
Lurbe, Empar
author_facet Martín de Llano, José Javier
Fuertes, Graciela
Torró, Isabel
García Vicent, Consuelo
Fayos, José Luis
Lurbe, Empar
author_sort Martín de Llano, José Javier
collection PubMed
description BACKGROUND: Low birth weight has been related to an increased risk for developing high blood pressure in adult life. The molecular and cellular analysis of umbilical cord artery and vein may provide information about the early vascular characteristics of an individual. We have assessed several phenotype characteristics of the four vascular cell types derived from human umbilical cords of newborns with different birth weight. Further follow-up studies could show the association of those vascular properties with infancy and adulthood blood pressure. METHODS: Endothelial and smooth muscle cell cultures were obtained from umbilical cords from two groups of newborns of birth weight less than 2.8 kg or higher than 3.5 kg. The expression of specific endothelial cell markers (von Willebrand factor, CD31, and the binding and internalization of acetylated low-density lipoprotein) and the smooth muscle cell specific α-actin have been evaluated. Cell culture viability, proliferation kinetic, growth fraction (expression of Ki67) and percentage of senescent cells (detection of β-galactosidase activity at pH 6.0) have been determined. Endothelial cell projection area was determined by morphometric analysis of cell cultures after CD31 immunodetection. RESULTS: The highest variation was found in cell density at the confluence of endothelial cell cultures derived from umbilical cord arteries (66,789 ± 5,093 cells/cm(2 )vs. 45,630 ± 11,927 cells/cm(2), p < 0.05). Morphometric analysis indicated that the projection area of the artery endothelial cells (1,161 ± 198 and 1,544 ± 472 μm(2), p < 0.05), but not those derived from the vein from individuals with a birth weight lower than 2.8 kg was lower than that of cells from individuals with a birth weight higher than 3.5 kg. CONCLUSION: The analysis of umbilical cord artery endothelial cells, which demonstrated differences in cell size related to birth weight, can provide hints about the cellular and molecular links between lower birth weight and increased adult high blood pressure risk.
format Text
id pubmed-2680391
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-26803912009-05-12 Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels Martín de Llano, José Javier Fuertes, Graciela Torró, Isabel García Vicent, Consuelo Fayos, José Luis Lurbe, Empar J Transl Med Research BACKGROUND: Low birth weight has been related to an increased risk for developing high blood pressure in adult life. The molecular and cellular analysis of umbilical cord artery and vein may provide information about the early vascular characteristics of an individual. We have assessed several phenotype characteristics of the four vascular cell types derived from human umbilical cords of newborns with different birth weight. Further follow-up studies could show the association of those vascular properties with infancy and adulthood blood pressure. METHODS: Endothelial and smooth muscle cell cultures were obtained from umbilical cords from two groups of newborns of birth weight less than 2.8 kg or higher than 3.5 kg. The expression of specific endothelial cell markers (von Willebrand factor, CD31, and the binding and internalization of acetylated low-density lipoprotein) and the smooth muscle cell specific α-actin have been evaluated. Cell culture viability, proliferation kinetic, growth fraction (expression of Ki67) and percentage of senescent cells (detection of β-galactosidase activity at pH 6.0) have been determined. Endothelial cell projection area was determined by morphometric analysis of cell cultures after CD31 immunodetection. RESULTS: The highest variation was found in cell density at the confluence of endothelial cell cultures derived from umbilical cord arteries (66,789 ± 5,093 cells/cm(2 )vs. 45,630 ± 11,927 cells/cm(2), p < 0.05). Morphometric analysis indicated that the projection area of the artery endothelial cells (1,161 ± 198 and 1,544 ± 472 μm(2), p < 0.05), but not those derived from the vein from individuals with a birth weight lower than 2.8 kg was lower than that of cells from individuals with a birth weight higher than 3.5 kg. CONCLUSION: The analysis of umbilical cord artery endothelial cells, which demonstrated differences in cell size related to birth weight, can provide hints about the cellular and molecular links between lower birth weight and increased adult high blood pressure risk. BioMed Central 2009-04-24 /pmc/articles/PMC2680391/ /pubmed/19393064 http://dx.doi.org/10.1186/1479-5876-7-30 Text en Copyright © 2009 Martín de Llano et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Martín de Llano, José Javier
Fuertes, Graciela
Torró, Isabel
García Vicent, Consuelo
Fayos, José Luis
Lurbe, Empar
Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels
title Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels
title_full Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels
title_fullStr Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels
title_full_unstemmed Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels
title_short Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels
title_sort birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680391/
https://www.ncbi.nlm.nih.gov/pubmed/19393064
http://dx.doi.org/10.1186/1479-5876-7-30
work_keys_str_mv AT martindellanojosejavier birthweightandcharacteristicsofendothelialandsmoothmusclecellculturesfromhumanumbilicalcordvessels
AT fuertesgraciela birthweightandcharacteristicsofendothelialandsmoothmusclecellculturesfromhumanumbilicalcordvessels
AT torroisabel birthweightandcharacteristicsofendothelialandsmoothmusclecellculturesfromhumanumbilicalcordvessels
AT garciavicentconsuelo birthweightandcharacteristicsofendothelialandsmoothmusclecellculturesfromhumanumbilicalcordvessels
AT fayosjoseluis birthweightandcharacteristicsofendothelialandsmoothmusclecellculturesfromhumanumbilicalcordvessels
AT lurbeempar birthweightandcharacteristicsofendothelialandsmoothmusclecellculturesfromhumanumbilicalcordvessels