Cargando…

Data-driven normalization strategies for high-throughput quantitative RT-PCR

BACKGROUND: High-throughput real-time quantitative reverse transcriptase polymerase chain reaction (qPCR) is a widely used technique in experiments where expression patterns of genes are to be profiled. Current stage technology allows the acquisition of profiles for a moderate number of genes (50 to...

Descripción completa

Detalles Bibliográficos
Autores principales:	Mar, Jessica C, Kimura, Yasumasa, Schroder, Kate, Irvine, Katharine M, Hayashizaki, Yoshihide, Suzuki, Harukazu, Hume, David, Quackenbush, John
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680405/ https://www.ncbi.nlm.nih.gov/pubmed/19374774 http://dx.doi.org/10.1186/1471-2105-10-110

Ejemplares similares

Development of a high-throughput method for the systematic identification of human proteins nuclear translocation potential
por: Hoat, Trinh Xuan, et al.
Publicado: (2009)

Selection of optimal reference genes for normalization in quantitative RT-PCR
por: Chervoneva, Inna, et al.
Publicado: (2010)

A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors
por: Caldana, Camila, et al.
Publicado: (2007)

A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in Medicago truncatula
por: Kakar, Klementina, et al.
Publicado: (2008)

A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV)
por: Liu, Wenwen, et al.
Publicado: (2013)

Human genome research enters a new phase
por: Suzuki, Harukazu, et al.
Publicado: (2005)

Semiquantitative RT-PCR measurement of gene expression in rat tissues including a correction for varying cell size and number
por: Romero, Maria del Mar, et al.
Publicado: (2007)

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
por: Balcells, Ingrid, et al.
Publicado: (2011)

Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR
por: Zhang, Ni, et al.
Publicado: (2011)

RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization
por: Hruz, Tomas, et al.
Publicado: (2011)

Simple regression for correcting ΔC(t) bias in RT-qPCR low-density array data normalization
por: Cui, Xiangqin, et al.
Publicado: (2015)

Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT-PCR)
por: Provenzano, Maurizio, et al.
Publicado: (2003)

Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I
por: Rodríguez-Mallon, Alina, et al.
Publicado: (2009)

Update of the FANTOM web resource: from mammalian transcriptional landscape to its dynamic regulation
por: Kawaji, Hideya, et al.
Publicado: (2011)

Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR
por: Su, Yi, et al.
Publicado: (2018)

A systemic approach to screening high-throughput RT-qPCR data for a suitable set of reference circulating miRNAs
por: Pagacz, Konrad, et al.
Publicado: (2020)

Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI
por: Bester, Rachelle, et al.
Publicado: (2012)

Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos
por: Kuijk, Ewart W, et al.
Publicado: (2007)

Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
por: Valente, Valeria, et al.
Publicado: (2009)

A quantitative real-time RT-PCR assay for mature C. albicans biofilms
por: Xie, Zhihong, et al.
Publicado: (2011)

The FANTOM web resource: from mammalian transcriptional landscape to its dynamic regulation
por: Kawaji, Hideya, et al.
Publicado: (2009)

Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
por: Chen, Canbin, et al.
Publicado: (2019)

Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs
por: Varkonyi-Gasic, Erika, et al.
Publicado: (2007)

Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses
por: Lu, Huanhuan, et al.
Publicado: (2021)

Suitable housekeeping genes for normalization of transcript abundance analysis by real-time RT-PCR in cultured bovine granulosa cells during hypoxia and differential cell plating density
por: Baddela, Vijay S, et al.
Publicado: (2014)

Simultaneous detection and differentiation of Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) by duplex real time RT-PCR
por: Zhang, Peng, et al.
Publicado: (2013)

Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
por: Long, Samuel, et al.
Publicado: (2021)

Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis
por: Chen, Yongxin, et al.
Publicado: (2009)

Ecological comparison of cellular stress responses among populations – normalizing RT-qPCR values to investigate differential environmental adaptations
por: Koenigstein, Stefan, et al.
Publicado: (2013)

Real-time RT-PCR assay to detect Granada virus and the related Massilia and Arrabida phleboviruses
por: Davó, Laura, et al.
Publicado: (2020)

A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots
por: Mounier, Thibault, et al.
Publicado: (2020)

Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers
por: Maroniche, Guillermo A, et al.
Publicado: (2011)

Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR
por: Kuamsab, Napaporn, et al.
Publicado: (2012)

Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses
por: Saxena, Parag, et al.
Publicado: (2008)

A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition
por: Scipioni, Alexandra, et al.
Publicado: (2008)

Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies
por: Noutsias, Michel, et al.
Publicado: (2008)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

A plastome primer set for comprehensive quantitative real time RT-PCR analysis of Zea mays: a starter primer set for other Poaceae species
por: Sharpe, Richard M, et al.
Publicado: (2008)

In vivo quantification of formulated and chemically modified small interfering RNA by heating-in-Triton quantitative reverse transcription polymerase chain reaction (HIT qRT-PCR)
por: Landesman, Yosef, et al.
Publicado: (2010)

Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays
por: Kimura, Yasumasa, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_eck8gvch06k6fo9kihf4510683