A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

BACKGROUND: Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications. FINDINGS: We describe here protocols which adapt baculovirus generation into 96-well format. CONCLUSION: The established meth...

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Autores principales: Kärkkäinen, Hanna-Riikka, Lesch, Hanna P, Määttä, Antti I, Toivanen, Pyry I, Mähönen, Anssi J, Roschier, Miia M, Airenne, Kari J, Laitinen, Olli H, Ylä-Herttuala, Seppo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680411/
https://www.ncbi.nlm.nih.gov/pubmed/19389242
http://dx.doi.org/10.1186/1756-0500-2-63
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author Kärkkäinen, Hanna-Riikka
Lesch, Hanna P
Määttä, Antti I
Toivanen, Pyry I
Mähönen, Anssi J
Roschier, Miia M
Airenne, Kari J
Laitinen, Olli H
Ylä-Herttuala, Seppo
author_facet Kärkkäinen, Hanna-Riikka
Lesch, Hanna P
Määttä, Antti I
Toivanen, Pyry I
Mähönen, Anssi J
Roschier, Miia M
Airenne, Kari J
Laitinen, Olli H
Ylä-Herttuala, Seppo
author_sort Kärkkäinen, Hanna-Riikka
collection PubMed
description BACKGROUND: Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications. FINDINGS: We describe here protocols which adapt baculovirus generation into 96-well format. CONCLUSION: The established methodology allows simple baculovirus generation, fast virus titering within 18 h and efficient recombinant protein production in a high-throughput format. Furthermore, the produced baculovirus vectors are compatible with gene expression in vertebrate cells in vitro and in vivo.
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spelling pubmed-26804112009-05-12 A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells Kärkkäinen, Hanna-Riikka Lesch, Hanna P Määttä, Antti I Toivanen, Pyry I Mähönen, Anssi J Roschier, Miia M Airenne, Kari J Laitinen, Olli H Ylä-Herttuala, Seppo BMC Res Notes Technical Note BACKGROUND: Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications. FINDINGS: We describe here protocols which adapt baculovirus generation into 96-well format. CONCLUSION: The established methodology allows simple baculovirus generation, fast virus titering within 18 h and efficient recombinant protein production in a high-throughput format. Furthermore, the produced baculovirus vectors are compatible with gene expression in vertebrate cells in vitro and in vivo. BioMed Central 2009-04-23 /pmc/articles/PMC2680411/ /pubmed/19389242 http://dx.doi.org/10.1186/1756-0500-2-63 Text en Copyright © 2009 Ylä-Herttuala et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Note
Kärkkäinen, Hanna-Riikka
Lesch, Hanna P
Määttä, Antti I
Toivanen, Pyry I
Mähönen, Anssi J
Roschier, Miia M
Airenne, Kari J
Laitinen, Olli H
Ylä-Herttuala, Seppo
A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells
title A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells
title_full A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells
title_fullStr A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells
title_full_unstemmed A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells
title_short A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells
title_sort 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680411/
https://www.ncbi.nlm.nih.gov/pubmed/19389242
http://dx.doi.org/10.1186/1756-0500-2-63
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