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Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide
We have investigated the pathway by which unilamellar POPC liposomes upon adsorption undergo rupture and form a supported lipid bilayer (SLB) on a SiO(2) surface. Biotinylated lipids were selectively incorporated in the outer monolayer of POPC liposomes to create liposomes with asymmetric lipid comp...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Molecular Diversity Preservation International (MDPI)
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680641/ https://www.ncbi.nlm.nih.gov/pubmed/19468333 http://dx.doi.org/10.3390/ijms10041683 |
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author | Reimhult, Erik Kasemo, Bengt Höök, Fredrik |
author_facet | Reimhult, Erik Kasemo, Bengt Höök, Fredrik |
author_sort | Reimhult, Erik |
collection | PubMed |
description | We have investigated the pathway by which unilamellar POPC liposomes upon adsorption undergo rupture and form a supported lipid bilayer (SLB) on a SiO(2) surface. Biotinylated lipids were selectively incorporated in the outer monolayer of POPC liposomes to create liposomes with asymmetric lipid compositions in the outer and inner leaflets. The specific binding of neutravidin and anti-biotin to SLBs formed by liposome fusion, prior to and after equilibrated flip-flop between the upper and lower monolayers in the SLB, were then investigated. It was concluded that the lipids in the outer monolayer of the vesicle predominantly end up on the SLB side facing the SiO(2) substrate, as demonstrated by having maximum 30–40% of lipids in the liposome outer monolayer orienting towards the bulk after forming the SLB. |
format | Text |
id | pubmed-2680641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-26806412009-05-22 Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide Reimhult, Erik Kasemo, Bengt Höök, Fredrik Int J Mol Sci Communication We have investigated the pathway by which unilamellar POPC liposomes upon adsorption undergo rupture and form a supported lipid bilayer (SLB) on a SiO(2) surface. Biotinylated lipids were selectively incorporated in the outer monolayer of POPC liposomes to create liposomes with asymmetric lipid compositions in the outer and inner leaflets. The specific binding of neutravidin and anti-biotin to SLBs formed by liposome fusion, prior to and after equilibrated flip-flop between the upper and lower monolayers in the SLB, were then investigated. It was concluded that the lipids in the outer monolayer of the vesicle predominantly end up on the SLB side facing the SiO(2) substrate, as demonstrated by having maximum 30–40% of lipids in the liposome outer monolayer orienting towards the bulk after forming the SLB. Molecular Diversity Preservation International (MDPI) 2009-04-17 /pmc/articles/PMC2680641/ /pubmed/19468333 http://dx.doi.org/10.3390/ijms10041683 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Communication Reimhult, Erik Kasemo, Bengt Höök, Fredrik Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide |
title | Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide |
title_full | Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide |
title_fullStr | Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide |
title_full_unstemmed | Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide |
title_short | Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide |
title_sort | rupture pathway of phosphatidylcholine liposomes on silicon dioxide |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680641/ https://www.ncbi.nlm.nih.gov/pubmed/19468333 http://dx.doi.org/10.3390/ijms10041683 |
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