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Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide

We have investigated the pathway by which unilamellar POPC liposomes upon adsorption undergo rupture and form a supported lipid bilayer (SLB) on a SiO(2) surface. Biotinylated lipids were selectively incorporated in the outer monolayer of POPC liposomes to create liposomes with asymmetric lipid comp...

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Detalles Bibliográficos
Autores principales: Reimhult, Erik, Kasemo, Bengt, Höök, Fredrik
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680641/
https://www.ncbi.nlm.nih.gov/pubmed/19468333
http://dx.doi.org/10.3390/ijms10041683
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author Reimhult, Erik
Kasemo, Bengt
Höök, Fredrik
author_facet Reimhult, Erik
Kasemo, Bengt
Höök, Fredrik
author_sort Reimhult, Erik
collection PubMed
description We have investigated the pathway by which unilamellar POPC liposomes upon adsorption undergo rupture and form a supported lipid bilayer (SLB) on a SiO(2) surface. Biotinylated lipids were selectively incorporated in the outer monolayer of POPC liposomes to create liposomes with asymmetric lipid compositions in the outer and inner leaflets. The specific binding of neutravidin and anti-biotin to SLBs formed by liposome fusion, prior to and after equilibrated flip-flop between the upper and lower monolayers in the SLB, were then investigated. It was concluded that the lipids in the outer monolayer of the vesicle predominantly end up on the SLB side facing the SiO(2) substrate, as demonstrated by having maximum 30–40% of lipids in the liposome outer monolayer orienting towards the bulk after forming the SLB.
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spelling pubmed-26806412009-05-22 Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide Reimhult, Erik Kasemo, Bengt Höök, Fredrik Int J Mol Sci Communication We have investigated the pathway by which unilamellar POPC liposomes upon adsorption undergo rupture and form a supported lipid bilayer (SLB) on a SiO(2) surface. Biotinylated lipids were selectively incorporated in the outer monolayer of POPC liposomes to create liposomes with asymmetric lipid compositions in the outer and inner leaflets. The specific binding of neutravidin and anti-biotin to SLBs formed by liposome fusion, prior to and after equilibrated flip-flop between the upper and lower monolayers in the SLB, were then investigated. It was concluded that the lipids in the outer monolayer of the vesicle predominantly end up on the SLB side facing the SiO(2) substrate, as demonstrated by having maximum 30–40% of lipids in the liposome outer monolayer orienting towards the bulk after forming the SLB. Molecular Diversity Preservation International (MDPI) 2009-04-17 /pmc/articles/PMC2680641/ /pubmed/19468333 http://dx.doi.org/10.3390/ijms10041683 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Reimhult, Erik
Kasemo, Bengt
Höök, Fredrik
Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide
title Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide
title_full Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide
title_fullStr Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide
title_full_unstemmed Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide
title_short Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide
title_sort rupture pathway of phosphatidylcholine liposomes on silicon dioxide
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680641/
https://www.ncbi.nlm.nih.gov/pubmed/19468333
http://dx.doi.org/10.3390/ijms10041683
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