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Non-Enzymatic Template-Directed Recombination of RNAs
RNA non-enzymatic recombination reactions are of great interest within the hypothesis of the “RNA world”, which argues that at some stage of prebiotic life development proteins were not yet engaged in biochemical reactions and RNA carried out both the information storage task and the full range of c...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680647/ https://www.ncbi.nlm.nih.gov/pubmed/19468339 http://dx.doi.org/10.3390/ijms10041788 |
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author | Nechaev, Sergey Y. Lutay, Alexei V. Vlassov, Valentin V. Zenkova, Marina A. |
author_facet | Nechaev, Sergey Y. Lutay, Alexei V. Vlassov, Valentin V. Zenkova, Marina A. |
author_sort | Nechaev, Sergey Y. |
collection | PubMed |
description | RNA non-enzymatic recombination reactions are of great interest within the hypothesis of the “RNA world”, which argues that at some stage of prebiotic life development proteins were not yet engaged in biochemical reactions and RNA carried out both the information storage task and the full range of catalytic roles necessary in primitive self-replicating systems. Here we report on the study of recombination reaction occuring between two 96 nucleotides (nts) fragments of RNAs under physiological conditions and governed by a short oligodeoxyribonucleotide template, partially complementary to sequences within each of the RNAs. Analysis of recombination products shows that ligation is predominantly template-directed, and occurs within the complementary complex with the template in “butt-to-butt” manner, in 1- or 3- nts bulges or in 2–3 nts internal loops. Minor recombination products formed in the template-independent manner are detected as well. |
format | Text |
id | pubmed-2680647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-26806472009-05-22 Non-Enzymatic Template-Directed Recombination of RNAs Nechaev, Sergey Y. Lutay, Alexei V. Vlassov, Valentin V. Zenkova, Marina A. Int J Mol Sci Article RNA non-enzymatic recombination reactions are of great interest within the hypothesis of the “RNA world”, which argues that at some stage of prebiotic life development proteins were not yet engaged in biochemical reactions and RNA carried out both the information storage task and the full range of catalytic roles necessary in primitive self-replicating systems. Here we report on the study of recombination reaction occuring between two 96 nucleotides (nts) fragments of RNAs under physiological conditions and governed by a short oligodeoxyribonucleotide template, partially complementary to sequences within each of the RNAs. Analysis of recombination products shows that ligation is predominantly template-directed, and occurs within the complementary complex with the template in “butt-to-butt” manner, in 1- or 3- nts bulges or in 2–3 nts internal loops. Minor recombination products formed in the template-independent manner are detected as well. Molecular Diversity Preservation International (MDPI) 2009-04-21 /pmc/articles/PMC2680647/ /pubmed/19468339 http://dx.doi.org/10.3390/ijms10041788 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Nechaev, Sergey Y. Lutay, Alexei V. Vlassov, Valentin V. Zenkova, Marina A. Non-Enzymatic Template-Directed Recombination of RNAs |
title | Non-Enzymatic Template-Directed Recombination of RNAs |
title_full | Non-Enzymatic Template-Directed Recombination of RNAs |
title_fullStr | Non-Enzymatic Template-Directed Recombination of RNAs |
title_full_unstemmed | Non-Enzymatic Template-Directed Recombination of RNAs |
title_short | Non-Enzymatic Template-Directed Recombination of RNAs |
title_sort | non-enzymatic template-directed recombination of rnas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680647/ https://www.ncbi.nlm.nih.gov/pubmed/19468339 http://dx.doi.org/10.3390/ijms10041788 |
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