Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays

Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime...

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Detalles Bibliográficos
Autores principales: Giraud, Gerard, Schulze, Holger, Bachmann, Till T., Campbell, Colin J., R., Andrew, Mount, Ghazal, Peter, Khondoker, Mizanur R., Ross, Alan J., Ember, Stuart W. J., Ciani, Ilenia, Tlili, Chaker, Walton, Anthony J., Terry, Jonathan G., Crain, Jason
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680655/
https://www.ncbi.nlm.nih.gov/pubmed/19468347
http://dx.doi.org/10.3390/ijms10041930
Descripción
Sumario:Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.

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