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Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays
Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Molecular Diversity Preservation International (MDPI)
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680655/ https://www.ncbi.nlm.nih.gov/pubmed/19468347 http://dx.doi.org/10.3390/ijms10041930 |
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| author | Giraud, Gerard Schulze, Holger Bachmann, Till T. Campbell, Colin J. R., Andrew Mount, Ghazal, Peter Khondoker, Mizanur R. Ross, Alan J. Ember, Stuart W. J. Ciani, Ilenia Tlili, Chaker Walton, Anthony J. Terry, Jonathan G. Crain, Jason |
| author_facet | Giraud, Gerard Schulze, Holger Bachmann, Till T. Campbell, Colin J. R., Andrew Mount, Ghazal, Peter Khondoker, Mizanur R. Ross, Alan J. Ember, Stuart W. J. Ciani, Ilenia Tlili, Chaker Walton, Anthony J. Terry, Jonathan G. Crain, Jason |
| author_sort | Giraud, Gerard |
| collection | PubMed |
| description | Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel. |
| format | Text |
| id | pubmed-2680655 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2009 |
| publisher | Molecular Diversity Preservation International (MDPI) |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-26806552009-05-22 Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays Giraud, Gerard Schulze, Holger Bachmann, Till T. Campbell, Colin J. R., Andrew Mount, Ghazal, Peter Khondoker, Mizanur R. Ross, Alan J. Ember, Stuart W. J. Ciani, Ilenia Tlili, Chaker Walton, Anthony J. Terry, Jonathan G. Crain, Jason Int J Mol Sci Article Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel. Molecular Diversity Preservation International (MDPI) 2009-04-24 /pmc/articles/PMC2680655/ /pubmed/19468347 http://dx.doi.org/10.3390/ijms10041930 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
| spellingShingle | Article Giraud, Gerard Schulze, Holger Bachmann, Till T. Campbell, Colin J. R., Andrew Mount, Ghazal, Peter Khondoker, Mizanur R. Ross, Alan J. Ember, Stuart W. J. Ciani, Ilenia Tlili, Chaker Walton, Anthony J. Terry, Jonathan G. Crain, Jason Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays |
| title | Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays |
| title_full | Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays |
| title_fullStr | Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays |
| title_full_unstemmed | Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays |
| title_short | Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays |
| title_sort | fluorescence lifetime imaging of quantum dot labeled dna microarrays |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680655/ https://www.ncbi.nlm.nih.gov/pubmed/19468347 http://dx.doi.org/10.3390/ijms10041930 |
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