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Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues

BACKGROUND: The concept of metabolite profiling has been around for decades and technical innovations are now enabling it to be carried out on a large scale with respect to the number of both metabolites measured and experiments carried out. However, studies are generally confined to polar compounds...

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Autores principales: Lytovchenko, Anna, Beleggia, Romina, Schauer, Nicolas, Isaacson, Tal, Leuendorf, Jan E, Hellmann, Hanjo, Rose, Jocelyn KC, Fernie, Alisdair R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680844/
https://www.ncbi.nlm.nih.gov/pubmed/19393072
http://dx.doi.org/10.1186/1746-4811-5-4
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author Lytovchenko, Anna
Beleggia, Romina
Schauer, Nicolas
Isaacson, Tal
Leuendorf, Jan E
Hellmann, Hanjo
Rose, Jocelyn KC
Fernie, Alisdair R
author_facet Lytovchenko, Anna
Beleggia, Romina
Schauer, Nicolas
Isaacson, Tal
Leuendorf, Jan E
Hellmann, Hanjo
Rose, Jocelyn KC
Fernie, Alisdair R
author_sort Lytovchenko, Anna
collection PubMed
description BACKGROUND: The concept of metabolite profiling has been around for decades and technical innovations are now enabling it to be carried out on a large scale with respect to the number of both metabolites measured and experiments carried out. However, studies are generally confined to polar compounds alone. Here we describe a simple method for lipophilic compounds analysis in various plant tissues. RESULTS: We choose the same preparative and instrumental platform for lipophilic profiling as that we routinely use for polar metabolites measurements. The method was validated in terms of linearity, carryover, reproducibility and recovery rates, as well as using various plant tissues. As a first case study we present metabolic profiling of Arabidopsis root and shoot tissue of wild type (C24) and mutant (rsr4-1) plants deficient on vitamin B6. We found significant alterations in lipid constituent contents, especially in the roots, which were characterised by dramatic increases in several fatty acids, thus providing further hint for the role of pyridoxine in oxidative stress and lipid peroxidation. The second example is the lipophilic profiling of red and green tomato fruit cuticles of wild type (Alisa Craig) and the DFD (delayed fruit deterioration) mutant, which we compared and contrasted with the more focused wax analysis of these plants reported before. CONCLUSION: We can rapidly and reliably detect and quantify over 40 lipophilic metabolites including fatty acids, fatty alcohols, alkanes, sterols and tocopherols. The method presented here affords a simple and rapid, yet robust complement to previously validated methods of polar metabolite profiling by gas-chromatography mass-spectrometry.
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spelling pubmed-26808442009-05-13 Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues Lytovchenko, Anna Beleggia, Romina Schauer, Nicolas Isaacson, Tal Leuendorf, Jan E Hellmann, Hanjo Rose, Jocelyn KC Fernie, Alisdair R Plant Methods Research BACKGROUND: The concept of metabolite profiling has been around for decades and technical innovations are now enabling it to be carried out on a large scale with respect to the number of both metabolites measured and experiments carried out. However, studies are generally confined to polar compounds alone. Here we describe a simple method for lipophilic compounds analysis in various plant tissues. RESULTS: We choose the same preparative and instrumental platform for lipophilic profiling as that we routinely use for polar metabolites measurements. The method was validated in terms of linearity, carryover, reproducibility and recovery rates, as well as using various plant tissues. As a first case study we present metabolic profiling of Arabidopsis root and shoot tissue of wild type (C24) and mutant (rsr4-1) plants deficient on vitamin B6. We found significant alterations in lipid constituent contents, especially in the roots, which were characterised by dramatic increases in several fatty acids, thus providing further hint for the role of pyridoxine in oxidative stress and lipid peroxidation. The second example is the lipophilic profiling of red and green tomato fruit cuticles of wild type (Alisa Craig) and the DFD (delayed fruit deterioration) mutant, which we compared and contrasted with the more focused wax analysis of these plants reported before. CONCLUSION: We can rapidly and reliably detect and quantify over 40 lipophilic metabolites including fatty acids, fatty alcohols, alkanes, sterols and tocopherols. The method presented here affords a simple and rapid, yet robust complement to previously validated methods of polar metabolite profiling by gas-chromatography mass-spectrometry. BioMed Central 2009-04-24 /pmc/articles/PMC2680844/ /pubmed/19393072 http://dx.doi.org/10.1186/1746-4811-5-4 Text en Copyright © 2009 Lytovchenko et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Lytovchenko, Anna
Beleggia, Romina
Schauer, Nicolas
Isaacson, Tal
Leuendorf, Jan E
Hellmann, Hanjo
Rose, Jocelyn KC
Fernie, Alisdair R
Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues
title Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues
title_full Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues
title_fullStr Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues
title_full_unstemmed Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues
title_short Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues
title_sort application of gc-ms for the detection of lipophilic compounds in diverse plant tissues
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680844/
https://www.ncbi.nlm.nih.gov/pubmed/19393072
http://dx.doi.org/10.1186/1746-4811-5-4
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