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High abundances of neurotrophin 3 in atopic dermatitis mast cell
BACKGROUND: Neurotrophin 3 (NT-3) is a member of the neurotrophin family, a group of related proteins that are known to regulate neuro-immune interactions in allergic diseases. Their cellular sources and role in the recruitment of mast cell precursors in atopic dermatitis have not been characterized...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680865/ https://www.ncbi.nlm.nih.gov/pubmed/19386090 http://dx.doi.org/10.1186/1745-6673-4-8 |
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author | Quarcoo, David Fischer, Tanja C Peckenschneider, Nora Groneberg, David A Welker, Pia |
author_facet | Quarcoo, David Fischer, Tanja C Peckenschneider, Nora Groneberg, David A Welker, Pia |
author_sort | Quarcoo, David |
collection | PubMed |
description | BACKGROUND: Neurotrophin 3 (NT-3) is a member of the neurotrophin family, a group of related proteins that are known to regulate neuro-immune interactions in allergic diseases. Their cellular sources and role in the recruitment of mast cell precursors in atopic dermatitis have not been characterized in detail so far. OBJECTIVE: Characterize NT-3 on a transcriptional and translational level in individuals with atopic dermatitis with special focus on mast cells. METHODS: To meet this objective NT-3 levels in the serum of AD patients were measured, the effect of NT-3 on keratinocytes was evaluated and the gene expression and regulation assessed using ELISA, immunohistochemistry and RNA quantification. RESULTS: Systemic levels of NT-3 were found to be higher in individuals with AD as compared to healthy controls. A distinct genetic expression was found in the various cells of the skin. In lesional mast cells of individuals with atopic dermatitis an increased amount of NT-3 was apparent. Functional in vitro experiments demonstrated that NT-3 stimulation led to a suppression of IL-8 secretion by HaCat cells. CONCLUSION: These findings could imply a role for NT-3 in the pathogenesis of allergic skin diseases. |
format | Text |
id | pubmed-2680865 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26808652009-05-13 High abundances of neurotrophin 3 in atopic dermatitis mast cell Quarcoo, David Fischer, Tanja C Peckenschneider, Nora Groneberg, David A Welker, Pia J Occup Med Toxicol Research BACKGROUND: Neurotrophin 3 (NT-3) is a member of the neurotrophin family, a group of related proteins that are known to regulate neuro-immune interactions in allergic diseases. Their cellular sources and role in the recruitment of mast cell precursors in atopic dermatitis have not been characterized in detail so far. OBJECTIVE: Characterize NT-3 on a transcriptional and translational level in individuals with atopic dermatitis with special focus on mast cells. METHODS: To meet this objective NT-3 levels in the serum of AD patients were measured, the effect of NT-3 on keratinocytes was evaluated and the gene expression and regulation assessed using ELISA, immunohistochemistry and RNA quantification. RESULTS: Systemic levels of NT-3 were found to be higher in individuals with AD as compared to healthy controls. A distinct genetic expression was found in the various cells of the skin. In lesional mast cells of individuals with atopic dermatitis an increased amount of NT-3 was apparent. Functional in vitro experiments demonstrated that NT-3 stimulation led to a suppression of IL-8 secretion by HaCat cells. CONCLUSION: These findings could imply a role for NT-3 in the pathogenesis of allergic skin diseases. BioMed Central 2009-04-22 /pmc/articles/PMC2680865/ /pubmed/19386090 http://dx.doi.org/10.1186/1745-6673-4-8 Text en Copyright © 2009 Quarcoo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Quarcoo, David Fischer, Tanja C Peckenschneider, Nora Groneberg, David A Welker, Pia High abundances of neurotrophin 3 in atopic dermatitis mast cell |
title | High abundances of neurotrophin 3 in atopic dermatitis mast cell |
title_full | High abundances of neurotrophin 3 in atopic dermatitis mast cell |
title_fullStr | High abundances of neurotrophin 3 in atopic dermatitis mast cell |
title_full_unstemmed | High abundances of neurotrophin 3 in atopic dermatitis mast cell |
title_short | High abundances of neurotrophin 3 in atopic dermatitis mast cell |
title_sort | high abundances of neurotrophin 3 in atopic dermatitis mast cell |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680865/ https://www.ncbi.nlm.nih.gov/pubmed/19386090 http://dx.doi.org/10.1186/1745-6673-4-8 |
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