Cargando…

High abundances of neurotrophin 3 in atopic dermatitis mast cell

BACKGROUND: Neurotrophin 3 (NT-3) is a member of the neurotrophin family, a group of related proteins that are known to regulate neuro-immune interactions in allergic diseases. Their cellular sources and role in the recruitment of mast cell precursors in atopic dermatitis have not been characterized...

Descripción completa

Detalles Bibliográficos
Autores principales: Quarcoo, David, Fischer, Tanja C, Peckenschneider, Nora, Groneberg, David A, Welker, Pia
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680865/
https://www.ncbi.nlm.nih.gov/pubmed/19386090
http://dx.doi.org/10.1186/1745-6673-4-8
_version_ 1782166982296076288
author Quarcoo, David
Fischer, Tanja C
Peckenschneider, Nora
Groneberg, David A
Welker, Pia
author_facet Quarcoo, David
Fischer, Tanja C
Peckenschneider, Nora
Groneberg, David A
Welker, Pia
author_sort Quarcoo, David
collection PubMed
description BACKGROUND: Neurotrophin 3 (NT-3) is a member of the neurotrophin family, a group of related proteins that are known to regulate neuro-immune interactions in allergic diseases. Their cellular sources and role in the recruitment of mast cell precursors in atopic dermatitis have not been characterized in detail so far. OBJECTIVE: Characterize NT-3 on a transcriptional and translational level in individuals with atopic dermatitis with special focus on mast cells. METHODS: To meet this objective NT-3 levels in the serum of AD patients were measured, the effect of NT-3 on keratinocytes was evaluated and the gene expression and regulation assessed using ELISA, immunohistochemistry and RNA quantification. RESULTS: Systemic levels of NT-3 were found to be higher in individuals with AD as compared to healthy controls. A distinct genetic expression was found in the various cells of the skin. In lesional mast cells of individuals with atopic dermatitis an increased amount of NT-3 was apparent. Functional in vitro experiments demonstrated that NT-3 stimulation led to a suppression of IL-8 secretion by HaCat cells. CONCLUSION: These findings could imply a role for NT-3 in the pathogenesis of allergic skin diseases.
format Text
id pubmed-2680865
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-26808652009-05-13 High abundances of neurotrophin 3 in atopic dermatitis mast cell Quarcoo, David Fischer, Tanja C Peckenschneider, Nora Groneberg, David A Welker, Pia J Occup Med Toxicol Research BACKGROUND: Neurotrophin 3 (NT-3) is a member of the neurotrophin family, a group of related proteins that are known to regulate neuro-immune interactions in allergic diseases. Their cellular sources and role in the recruitment of mast cell precursors in atopic dermatitis have not been characterized in detail so far. OBJECTIVE: Characterize NT-3 on a transcriptional and translational level in individuals with atopic dermatitis with special focus on mast cells. METHODS: To meet this objective NT-3 levels in the serum of AD patients were measured, the effect of NT-3 on keratinocytes was evaluated and the gene expression and regulation assessed using ELISA, immunohistochemistry and RNA quantification. RESULTS: Systemic levels of NT-3 were found to be higher in individuals with AD as compared to healthy controls. A distinct genetic expression was found in the various cells of the skin. In lesional mast cells of individuals with atopic dermatitis an increased amount of NT-3 was apparent. Functional in vitro experiments demonstrated that NT-3 stimulation led to a suppression of IL-8 secretion by HaCat cells. CONCLUSION: These findings could imply a role for NT-3 in the pathogenesis of allergic skin diseases. BioMed Central 2009-04-22 /pmc/articles/PMC2680865/ /pubmed/19386090 http://dx.doi.org/10.1186/1745-6673-4-8 Text en Copyright © 2009 Quarcoo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Quarcoo, David
Fischer, Tanja C
Peckenschneider, Nora
Groneberg, David A
Welker, Pia
High abundances of neurotrophin 3 in atopic dermatitis mast cell
title High abundances of neurotrophin 3 in atopic dermatitis mast cell
title_full High abundances of neurotrophin 3 in atopic dermatitis mast cell
title_fullStr High abundances of neurotrophin 3 in atopic dermatitis mast cell
title_full_unstemmed High abundances of neurotrophin 3 in atopic dermatitis mast cell
title_short High abundances of neurotrophin 3 in atopic dermatitis mast cell
title_sort high abundances of neurotrophin 3 in atopic dermatitis mast cell
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680865/
https://www.ncbi.nlm.nih.gov/pubmed/19386090
http://dx.doi.org/10.1186/1745-6673-4-8
work_keys_str_mv AT quarcoodavid highabundancesofneurotrophin3inatopicdermatitismastcell
AT fischertanjac highabundancesofneurotrophin3inatopicdermatitismastcell
AT peckenschneidernora highabundancesofneurotrophin3inatopicdermatitismastcell
AT gronebergdavida highabundancesofneurotrophin3inatopicdermatitismastcell
AT welkerpia highabundancesofneurotrophin3inatopicdermatitismastcell