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Genetic dissection of MHC-associated susceptibility to Lepeophtheirus salmonis in Atlantic salmon
BACKGROUND: Genetic variation has been shown to play a significant role in determining susceptibility to the salmon louse, Lepeophtheirus salmonis. However, the mechanisms involved in differential response to infection remain poorly understood. Recent findings in Atlantic salmon (Salmo salar) have p...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680909/ https://www.ncbi.nlm.nih.gov/pubmed/19397823 http://dx.doi.org/10.1186/1471-2156-10-20 |
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author | Gharbi, Karim Glover, Kevin A Stone, Louise C MacDonald, Elizabeth S Matthews, Louise Grimholt, Unni Stear, Michael J |
author_facet | Gharbi, Karim Glover, Kevin A Stone, Louise C MacDonald, Elizabeth S Matthews, Louise Grimholt, Unni Stear, Michael J |
author_sort | Gharbi, Karim |
collection | PubMed |
description | BACKGROUND: Genetic variation has been shown to play a significant role in determining susceptibility to the salmon louse, Lepeophtheirus salmonis. However, the mechanisms involved in differential response to infection remain poorly understood. Recent findings in Atlantic salmon (Salmo salar) have provided evidence for a potential link between marker variation at the major histocompatibility complex (MHC) and differences in lice abundance among infected siblings, suggesting that MHC genes can modulate susceptibility to the parasite. In this study, we used quantitative trait locus (QTL) analysis to test the effect of genomic regions linked to MHC class I and II on linkage groups (LG) 15 and 6, respectively. RESULTS: Significant QTL effects were detected on both LG 6 and LG 15 in sire-based analysis but the QTL regions remained unresolved due to a lack of recombination between markers. In dam-based analysis, a significant QTL was identified on LG 6, which accounted for 12.9% of within-family variance in lice abundance. However, the QTL was located at the opposite end of DAA, with no significant overlap with the MHC class II region. Interestingly, QTL modelling also revealed evidence of sex-linked differences in lice abundance, indicating that males and females may have different susceptibility to infection. CONCLUSION: Overall, QTL analysis provided relatively weak support for a proximal effect of classical MHC regions on lice abundance, which can partly be explained by linkage to other genes controlling susceptibility to L. salmonis on the same chromosome. |
format | Text |
id | pubmed-2680909 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26809092009-05-13 Genetic dissection of MHC-associated susceptibility to Lepeophtheirus salmonis in Atlantic salmon Gharbi, Karim Glover, Kevin A Stone, Louise C MacDonald, Elizabeth S Matthews, Louise Grimholt, Unni Stear, Michael J BMC Genet Research Article BACKGROUND: Genetic variation has been shown to play a significant role in determining susceptibility to the salmon louse, Lepeophtheirus salmonis. However, the mechanisms involved in differential response to infection remain poorly understood. Recent findings in Atlantic salmon (Salmo salar) have provided evidence for a potential link between marker variation at the major histocompatibility complex (MHC) and differences in lice abundance among infected siblings, suggesting that MHC genes can modulate susceptibility to the parasite. In this study, we used quantitative trait locus (QTL) analysis to test the effect of genomic regions linked to MHC class I and II on linkage groups (LG) 15 and 6, respectively. RESULTS: Significant QTL effects were detected on both LG 6 and LG 15 in sire-based analysis but the QTL regions remained unresolved due to a lack of recombination between markers. In dam-based analysis, a significant QTL was identified on LG 6, which accounted for 12.9% of within-family variance in lice abundance. However, the QTL was located at the opposite end of DAA, with no significant overlap with the MHC class II region. Interestingly, QTL modelling also revealed evidence of sex-linked differences in lice abundance, indicating that males and females may have different susceptibility to infection. CONCLUSION: Overall, QTL analysis provided relatively weak support for a proximal effect of classical MHC regions on lice abundance, which can partly be explained by linkage to other genes controlling susceptibility to L. salmonis on the same chromosome. BioMed Central 2009-04-27 /pmc/articles/PMC2680909/ /pubmed/19397823 http://dx.doi.org/10.1186/1471-2156-10-20 Text en Copyright © 2009 Gharbi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gharbi, Karim Glover, Kevin A Stone, Louise C MacDonald, Elizabeth S Matthews, Louise Grimholt, Unni Stear, Michael J Genetic dissection of MHC-associated susceptibility to Lepeophtheirus salmonis in Atlantic salmon |
title | Genetic dissection of MHC-associated susceptibility to Lepeophtheirus salmonis in Atlantic salmon |
title_full | Genetic dissection of MHC-associated susceptibility to Lepeophtheirus salmonis in Atlantic salmon |
title_fullStr | Genetic dissection of MHC-associated susceptibility to Lepeophtheirus salmonis in Atlantic salmon |
title_full_unstemmed | Genetic dissection of MHC-associated susceptibility to Lepeophtheirus salmonis in Atlantic salmon |
title_short | Genetic dissection of MHC-associated susceptibility to Lepeophtheirus salmonis in Atlantic salmon |
title_sort | genetic dissection of mhc-associated susceptibility to lepeophtheirus salmonis in atlantic salmon |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680909/ https://www.ncbi.nlm.nih.gov/pubmed/19397823 http://dx.doi.org/10.1186/1471-2156-10-20 |
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