Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay

Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mic...

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Detalles Bibliográficos
Autores principales: Karapetyan, Yervand Eduard, Saá, Paula, Mahal, Sukhvir Paul, Sferrazza, Gian Franco, Sherman, Alexandra, Salès, Nicole, Weissmann, Charles, Lasmézas, Corinne Ida
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2684634/
https://www.ncbi.nlm.nih.gov/pubmed/19478942
http://dx.doi.org/10.1371/journal.pone.0005730
Descripción
Sumario:Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay.

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