Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay

Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mic...

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Autores principales: Karapetyan, Yervand Eduard, Saá, Paula, Mahal, Sukhvir Paul, Sferrazza, Gian Franco, Sherman, Alexandra, Salès, Nicole, Weissmann, Charles, Lasmézas, Corinne Ida
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2684634/
https://www.ncbi.nlm.nih.gov/pubmed/19478942
http://dx.doi.org/10.1371/journal.pone.0005730
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author Karapetyan, Yervand Eduard
Saá, Paula
Mahal, Sukhvir Paul
Sferrazza, Gian Franco
Sherman, Alexandra
Salès, Nicole
Weissmann, Charles
Lasmézas, Corinne Ida
author_facet Karapetyan, Yervand Eduard
Saá, Paula
Mahal, Sukhvir Paul
Sferrazza, Gian Franco
Sherman, Alexandra
Salès, Nicole
Weissmann, Charles
Lasmézas, Corinne Ida
author_sort Karapetyan, Yervand Eduard
collection PubMed
description Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay.
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spelling pubmed-26846342009-05-29 Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay Karapetyan, Yervand Eduard Saá, Paula Mahal, Sukhvir Paul Sferrazza, Gian Franco Sherman, Alexandra Salès, Nicole Weissmann, Charles Lasmézas, Corinne Ida PLoS One Research Article Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay. Public Library of Science 2009-05-29 /pmc/articles/PMC2684634/ /pubmed/19478942 http://dx.doi.org/10.1371/journal.pone.0005730 Text en Karapetyan et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Karapetyan, Yervand Eduard
Saá, Paula
Mahal, Sukhvir Paul
Sferrazza, Gian Franco
Sherman, Alexandra
Salès, Nicole
Weissmann, Charles
Lasmézas, Corinne Ida
Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay
title Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay
title_full Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay
title_fullStr Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay
title_full_unstemmed Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay
title_short Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay
title_sort prion strain discrimination based on rapid in vivo amplification and analysis by the cell panel assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2684634/
https://www.ncbi.nlm.nih.gov/pubmed/19478942
http://dx.doi.org/10.1371/journal.pone.0005730
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