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Modulation of NKT cells and Th1/Th2 imbalance after α-GalCer treatment in progressive load-trained rats

Purpose: The purpose of this study was to determine whether α-galactosylceramide (α-GalCer), a synthetic glycolipid agonist of natural killer T (NKT) cells, can ameliorate exercise-induced immune imbalance. Methods: Eight-week-old female Sprague-Dawley rats were trained with a progressively increasi...

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Autores principales: Ru, Wang, Peijie, Chen
Formato: Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2684679/
https://www.ncbi.nlm.nih.gov/pubmed/19461936
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author Ru, Wang
Peijie, Chen
author_facet Ru, Wang
Peijie, Chen
author_sort Ru, Wang
collection PubMed
description Purpose: The purpose of this study was to determine whether α-galactosylceramide (α-GalCer), a synthetic glycolipid agonist of natural killer T (NKT) cells, can ameliorate exercise-induced immune imbalance. Methods: Eight-week-old female Sprague-Dawley rats were trained with a progressively increasing load for 9 weeks. At 36 h and at 7 d after training, groups of rats were euthanized. The whole blood was used to detect hemoglobin(Hb), plasma was analyzed for hormones testosterone(T) and corticosterone(C), and spleen was harvested for detecting NKT cells and interferon-γ (IFN-γ) and interleukin (IL)-4 producing cells. Results: Two-way analysis of variance (ANOVA) showed significant differences between training and time in Series 1. The results showed, at 36h after training, that the decrease in Hb, T and C concentration reflected overtraining or excessive exercise. At 7 d after training, NKT cell populations decreased, and a T helper 1/T helper 2 (Th1/Th2) lymphocyte imbalance occurred. In Series 2, α-galactosylceramide (α-GalCer), an NKT cell activator was found to enhance NKT cell numbers by 69% and shift the Th1/Th2 lymphocyte imbalance by observably decreasing the frequency of IL-4 secreting cells. Conclusion: These data showed that, in addition to Th1/Th2 self-regulation, α-GalCer played an important modulatory role in the exercise-induced Th1/Th2 lymphocyte imbalance, which may be correlative with NKT immunoregulatory cells.
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spelling pubmed-26846792009-05-20 Modulation of NKT cells and Th1/Th2 imbalance after α-GalCer treatment in progressive load-trained rats Ru, Wang Peijie, Chen Int J Biol Sci Research Paper Purpose: The purpose of this study was to determine whether α-galactosylceramide (α-GalCer), a synthetic glycolipid agonist of natural killer T (NKT) cells, can ameliorate exercise-induced immune imbalance. Methods: Eight-week-old female Sprague-Dawley rats were trained with a progressively increasing load for 9 weeks. At 36 h and at 7 d after training, groups of rats were euthanized. The whole blood was used to detect hemoglobin(Hb), plasma was analyzed for hormones testosterone(T) and corticosterone(C), and spleen was harvested for detecting NKT cells and interferon-γ (IFN-γ) and interleukin (IL)-4 producing cells. Results: Two-way analysis of variance (ANOVA) showed significant differences between training and time in Series 1. The results showed, at 36h after training, that the decrease in Hb, T and C concentration reflected overtraining or excessive exercise. At 7 d after training, NKT cell populations decreased, and a T helper 1/T helper 2 (Th1/Th2) lymphocyte imbalance occurred. In Series 2, α-galactosylceramide (α-GalCer), an NKT cell activator was found to enhance NKT cell numbers by 69% and shift the Th1/Th2 lymphocyte imbalance by observably decreasing the frequency of IL-4 secreting cells. Conclusion: These data showed that, in addition to Th1/Th2 self-regulation, α-GalCer played an important modulatory role in the exercise-induced Th1/Th2 lymphocyte imbalance, which may be correlative with NKT immunoregulatory cells. Ivyspring International Publisher 2009-05-07 /pmc/articles/PMC2684679/ /pubmed/19461936 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Ru, Wang
Peijie, Chen
Modulation of NKT cells and Th1/Th2 imbalance after α-GalCer treatment in progressive load-trained rats
title Modulation of NKT cells and Th1/Th2 imbalance after α-GalCer treatment in progressive load-trained rats
title_full Modulation of NKT cells and Th1/Th2 imbalance after α-GalCer treatment in progressive load-trained rats
title_fullStr Modulation of NKT cells and Th1/Th2 imbalance after α-GalCer treatment in progressive load-trained rats
title_full_unstemmed Modulation of NKT cells and Th1/Th2 imbalance after α-GalCer treatment in progressive load-trained rats
title_short Modulation of NKT cells and Th1/Th2 imbalance after α-GalCer treatment in progressive load-trained rats
title_sort modulation of nkt cells and th1/th2 imbalance after α-galcer treatment in progressive load-trained rats
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2684679/
https://www.ncbi.nlm.nih.gov/pubmed/19461936
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