Improvement of In Situ PCR by Optimization of PCR Cycle Number and Proteinase K Concentration: Localization of X Chromosome-Linked Phosphoglycerate Kinase-1 Gene in Mouse Reproductive Organs

In situ polymerase chain reaction (in situ PCR), which can detect a few copies of genes within a cell by amplifying the target gene, was developed to better understand the biological functions of tissues. In this study, we optimized the protocol conditions for the detection of X chromosome-linked ph...

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Detalles Bibliográficos
Autores principales: Hishikawa, Yoshitaka, An, Shucai, Yamamoto-Fukuda, Tomomi, Shibata, Yasuaki, Koji, Takehiko
Formato: Texto
Lenguaje:English
Publicado: Japan Society of Histochemistry and Cytochemistry 2009
Materias:
Technical Advancement
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685019/
https://www.ncbi.nlm.nih.gov/pubmed/19492023
http://dx.doi.org/10.1267/ahc.09011

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685019/
https://www.ncbi.nlm.nih.gov/pubmed/19492023
http://dx.doi.org/10.1267/ahc.09011

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