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Localization of Adenylate Kinase 4 in Mouse Tissues
Adenylate kinase (AK) is a key enzyme in the high-energy phosphoryl transfer reaction in living cells. Of its isoforms, AK4 has a similar sequence and subcellular localization to that of AK3 in the mitochondrial matrix. However, unlike AK3, AK4 lacks the guanosine triphosphate: adenosine monophospha...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Japan Society of Histochemistry and Cytochemistry
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685024/ https://www.ncbi.nlm.nih.gov/pubmed/19492028 http://dx.doi.org/10.1267/ahc.08012 |
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author | Miyoshi, Keiko Akazawa, Yuki Horiguchi, Taigo Noma, Takafumi |
author_facet | Miyoshi, Keiko Akazawa, Yuki Horiguchi, Taigo Noma, Takafumi |
author_sort | Miyoshi, Keiko |
collection | PubMed |
description | Adenylate kinase (AK) is a key enzyme in the high-energy phosphoryl transfer reaction in living cells. Of its isoforms, AK4 has a similar sequence and subcellular localization to that of AK3 in the mitochondrial matrix. However, unlike AK3, AK4 lacks the guanosine triphosphate: adenosine monophosphate phosphotransferase activity. To elucidate the physiological role of AK4, we explored the protein localization of AK4 in various mouse tissues by immunohistochemical analysis. AK4 protein was detected in the kidney, liver, brain, heart, stomach, intestine, and gonads but not in the lung and spleen. Interestingly, cell-type specific expression was evident in the brain, gastrointestinal tract, and gonads. In the cerebellum, AK4 was detected in granular cells but not in Purkinje cell bodies. In the gastrointestinal tract, AK4 was highly expressed in epithelia. In the ovary, AK4 was detected in oocytes and corpora lutea. In the testis, AK4 was detected in spermatocytes but not in spermatogonia. Our findings demonstrate that AK4 localizes uniquely in a cell-type and tissue-specific manner in mouse tissues. |
format | Text |
id | pubmed-2685024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Japan Society of Histochemistry and Cytochemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-26850242009-06-02 Localization of Adenylate Kinase 4 in Mouse Tissues Miyoshi, Keiko Akazawa, Yuki Horiguchi, Taigo Noma, Takafumi Acta Histochem Cytochem Regular Article Adenylate kinase (AK) is a key enzyme in the high-energy phosphoryl transfer reaction in living cells. Of its isoforms, AK4 has a similar sequence and subcellular localization to that of AK3 in the mitochondrial matrix. However, unlike AK3, AK4 lacks the guanosine triphosphate: adenosine monophosphate phosphotransferase activity. To elucidate the physiological role of AK4, we explored the protein localization of AK4 in various mouse tissues by immunohistochemical analysis. AK4 protein was detected in the kidney, liver, brain, heart, stomach, intestine, and gonads but not in the lung and spleen. Interestingly, cell-type specific expression was evident in the brain, gastrointestinal tract, and gonads. In the cerebellum, AK4 was detected in granular cells but not in Purkinje cell bodies. In the gastrointestinal tract, AK4 was highly expressed in epithelia. In the ovary, AK4 was detected in oocytes and corpora lutea. In the testis, AK4 was detected in spermatocytes but not in spermatogonia. Our findings demonstrate that AK4 localizes uniquely in a cell-type and tissue-specific manner in mouse tissues. Japan Society of Histochemistry and Cytochemistry 2009-04-28 2009-04-07 /pmc/articles/PMC2685024/ /pubmed/19492028 http://dx.doi.org/10.1267/ahc.08012 Text en © 2009 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Article Miyoshi, Keiko Akazawa, Yuki Horiguchi, Taigo Noma, Takafumi Localization of Adenylate Kinase 4 in Mouse Tissues |
title | Localization of Adenylate Kinase 4 in Mouse Tissues |
title_full | Localization of Adenylate Kinase 4 in Mouse Tissues |
title_fullStr | Localization of Adenylate Kinase 4 in Mouse Tissues |
title_full_unstemmed | Localization of Adenylate Kinase 4 in Mouse Tissues |
title_short | Localization of Adenylate Kinase 4 in Mouse Tissues |
title_sort | localization of adenylate kinase 4 in mouse tissues |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685024/ https://www.ncbi.nlm.nih.gov/pubmed/19492028 http://dx.doi.org/10.1267/ahc.08012 |
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