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Effect of Quercetin on Preosteoblasts and Bone Defects
Quercetin is a flavonol, also a phytoestrogen, available commonly in onion and apple. Our laboratory investigated its effect on MC3T3-E1 cells’ alkaline phosphatase activity in vitro and compared the amount of new bone produced by quercetin in collagen matrix to that produced by bone grafts and coll...
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Formato: | Texto |
Lenguaje: | English |
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Bentham Open
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685048/ https://www.ncbi.nlm.nih.gov/pubmed/19461927 http://dx.doi.org/10.2174/1874325000802010027 |
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author | Wong, R.W.K Rabie, A.B.M |
author_facet | Wong, R.W.K Rabie, A.B.M |
author_sort | Wong, R.W.K |
collection | PubMed |
description | Quercetin is a flavonol, also a phytoestrogen, available commonly in onion and apple. Our laboratory investigated its effect on MC3T3-E1 cells’ alkaline phosphatase activity in vitro and compared the amount of new bone produced by quercetin in collagen matrix to that produced by bone grafts and collagen matrix in vivo. Four bone defects, 5mm by 10mm were created in the parietal bone of 2 New Zealand White rabbits. In the experimental animal, 2 defects were grafted with quercetin solution mixed with collagen matrix. In the control animal, 2 defects were grafted with collagen matrix alone. Animals were killed on day 14 and the defects were dissected and prepared for histological qualitative assessment. Results showed that 10μM of quercetin increased alkaline phosphatase activity of MC3T3-E1 cells at 72 hours in vitro by 32%. In the experimental animal, there was new bone growing inside the bone defects. In conclusion, specific concentration of quercetin increased alkaline phosphatase activity of MC3T3-E1 cells in vitro and quercetin in collagen matrix has the effect of forming new bone across bone defects in vivo. |
format | Text |
id | pubmed-2685048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Bentham Open |
record_format | MEDLINE/PubMed |
spelling | pubmed-26850482009-05-21 Effect of Quercetin on Preosteoblasts and Bone Defects Wong, R.W.K Rabie, A.B.M Open Orthop J Article Quercetin is a flavonol, also a phytoestrogen, available commonly in onion and apple. Our laboratory investigated its effect on MC3T3-E1 cells’ alkaline phosphatase activity in vitro and compared the amount of new bone produced by quercetin in collagen matrix to that produced by bone grafts and collagen matrix in vivo. Four bone defects, 5mm by 10mm were created in the parietal bone of 2 New Zealand White rabbits. In the experimental animal, 2 defects were grafted with quercetin solution mixed with collagen matrix. In the control animal, 2 defects were grafted with collagen matrix alone. Animals were killed on day 14 and the defects were dissected and prepared for histological qualitative assessment. Results showed that 10μM of quercetin increased alkaline phosphatase activity of MC3T3-E1 cells at 72 hours in vitro by 32%. In the experimental animal, there was new bone growing inside the bone defects. In conclusion, specific concentration of quercetin increased alkaline phosphatase activity of MC3T3-E1 cells in vitro and quercetin in collagen matrix has the effect of forming new bone across bone defects in vivo. Bentham Open 2008-03-10 /pmc/articles/PMC2685048/ /pubmed/19461927 http://dx.doi.org/10.2174/1874325000802010027 Text en © Wong and Rabie; Licensee Bentham Open. http://creativecommons.org/licenses/by/2.5/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Wong, R.W.K Rabie, A.B.M Effect of Quercetin on Preosteoblasts and Bone Defects |
title | Effect of Quercetin on Preosteoblasts and Bone Defects |
title_full | Effect of Quercetin on Preosteoblasts and Bone Defects |
title_fullStr | Effect of Quercetin on Preosteoblasts and Bone Defects |
title_full_unstemmed | Effect of Quercetin on Preosteoblasts and Bone Defects |
title_short | Effect of Quercetin on Preosteoblasts and Bone Defects |
title_sort | effect of quercetin on preosteoblasts and bone defects |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685048/ https://www.ncbi.nlm.nih.gov/pubmed/19461927 http://dx.doi.org/10.2174/1874325000802010027 |
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