Cargando…
The components of the Daphnia pulex immune system as revealed by complete genome sequencing
BACKGROUND: Branchiopod crustaceans in the genus Daphnia are key model organisms for investigating interactions between genes and the environment. One major theme of research on Daphnia species has been the evolution of resistance to pathogens and parasites, but lack of knowledge of the Daphnia immu...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685406/ https://www.ncbi.nlm.nih.gov/pubmed/19386092 http://dx.doi.org/10.1186/1471-2164-10-175 |
_version_ | 1782167323140947968 |
---|---|
author | McTaggart, Seanna J Conlon, Claire Colbourne, John K Blaxter, Mark L Little, Tom J |
author_facet | McTaggart, Seanna J Conlon, Claire Colbourne, John K Blaxter, Mark L Little, Tom J |
author_sort | McTaggart, Seanna J |
collection | PubMed |
description | BACKGROUND: Branchiopod crustaceans in the genus Daphnia are key model organisms for investigating interactions between genes and the environment. One major theme of research on Daphnia species has been the evolution of resistance to pathogens and parasites, but lack of knowledge of the Daphnia immune system has limited the study of immune responses. Here we provide a survey of the immune-related genome of D. pulex, derived from the newly completed genome sequence. Genes likely to be involved in innate immune responses were identified by comparison to homologues from other arthropods. For each candidate, the gene model was refined, and we conducted an analysis of sequence divergence from homologues from other taxa. RESULTS AND CONCLUSION: We found that some immune pathways, in particular the TOLL pathway, are fairly well conserved between insects and Daphnia, while other elements, in particular antimicrobial peptides, could not be recovered from the genome sequence. We also found considerable variation in gene family copy number when comparing Daphnia to insects and present phylogenetic analyses to shed light on the evolution of a range of conserved immune gene families. |
format | Text |
id | pubmed-2685406 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26854062009-05-22 The components of the Daphnia pulex immune system as revealed by complete genome sequencing McTaggart, Seanna J Conlon, Claire Colbourne, John K Blaxter, Mark L Little, Tom J BMC Genomics Research Article BACKGROUND: Branchiopod crustaceans in the genus Daphnia are key model organisms for investigating interactions between genes and the environment. One major theme of research on Daphnia species has been the evolution of resistance to pathogens and parasites, but lack of knowledge of the Daphnia immune system has limited the study of immune responses. Here we provide a survey of the immune-related genome of D. pulex, derived from the newly completed genome sequence. Genes likely to be involved in innate immune responses were identified by comparison to homologues from other arthropods. For each candidate, the gene model was refined, and we conducted an analysis of sequence divergence from homologues from other taxa. RESULTS AND CONCLUSION: We found that some immune pathways, in particular the TOLL pathway, are fairly well conserved between insects and Daphnia, while other elements, in particular antimicrobial peptides, could not be recovered from the genome sequence. We also found considerable variation in gene family copy number when comparing Daphnia to insects and present phylogenetic analyses to shed light on the evolution of a range of conserved immune gene families. BioMed Central 2009-04-22 /pmc/articles/PMC2685406/ /pubmed/19386092 http://dx.doi.org/10.1186/1471-2164-10-175 Text en Copyright © 2009 McTaggart et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article McTaggart, Seanna J Conlon, Claire Colbourne, John K Blaxter, Mark L Little, Tom J The components of the Daphnia pulex immune system as revealed by complete genome sequencing |
title | The components of the Daphnia pulex immune system as revealed by complete genome sequencing |
title_full | The components of the Daphnia pulex immune system as revealed by complete genome sequencing |
title_fullStr | The components of the Daphnia pulex immune system as revealed by complete genome sequencing |
title_full_unstemmed | The components of the Daphnia pulex immune system as revealed by complete genome sequencing |
title_short | The components of the Daphnia pulex immune system as revealed by complete genome sequencing |
title_sort | components of the daphnia pulex immune system as revealed by complete genome sequencing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685406/ https://www.ncbi.nlm.nih.gov/pubmed/19386092 http://dx.doi.org/10.1186/1471-2164-10-175 |
work_keys_str_mv | AT mctaggartseannaj thecomponentsofthedaphniapuleximmunesystemasrevealedbycompletegenomesequencing AT conlonclaire thecomponentsofthedaphniapuleximmunesystemasrevealedbycompletegenomesequencing AT colbournejohnk thecomponentsofthedaphniapuleximmunesystemasrevealedbycompletegenomesequencing AT blaxtermarkl thecomponentsofthedaphniapuleximmunesystemasrevealedbycompletegenomesequencing AT littletomj thecomponentsofthedaphniapuleximmunesystemasrevealedbycompletegenomesequencing AT mctaggartseannaj componentsofthedaphniapuleximmunesystemasrevealedbycompletegenomesequencing AT conlonclaire componentsofthedaphniapuleximmunesystemasrevealedbycompletegenomesequencing AT colbournejohnk componentsofthedaphniapuleximmunesystemasrevealedbycompletegenomesequencing AT blaxtermarkl componentsofthedaphniapuleximmunesystemasrevealedbycompletegenomesequencing AT littletomj componentsofthedaphniapuleximmunesystemasrevealedbycompletegenomesequencing |