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Changes in Glutamate/NMDA Receptor Subunit 1 Expression in Rat Brain after Acute and Subacute Exposure to Methamphetamine
Methamphetamine (METH) is a psychostimulant drug of abuse that produces long-term behavioral changes including behavioral sensitization, tolerance, and dependence. METH has been reported to induce neurotoxic effects in several areas of the brain via the dopaminergic system. Changes of dopamine funct...
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2686235/ https://www.ncbi.nlm.nih.gov/pubmed/19478962 http://dx.doi.org/10.1155/2009/329631 |
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author | Kerdsan, Walailuk Thanoi, Samur Nudmamud-Thanoi, Sutisa |
author_facet | Kerdsan, Walailuk Thanoi, Samur Nudmamud-Thanoi, Sutisa |
author_sort | Kerdsan, Walailuk |
collection | PubMed |
description | Methamphetamine (METH) is a psychostimulant drug of abuse that produces long-term behavioral changes including behavioral sensitization, tolerance, and dependence. METH has been reported to induce neurotoxic effects in several areas of the brain via the dopaminergic system. Changes of dopamine function can induce malfunction of the glutamatergic system. Therefore, the aim of the present study was to examine the effects of METH administration on the expression of glutamate N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in frontal cortex, striatum, and hippocampal formation after acute and subacute exposure to METH by western blotting. Male Sprague-Dawley rats were injected intraperitoneally with a single dose of 8 mg/kg METH, 4 mg/kg/day METH for 14 days and saline in acute, subacute, and control groups, respectively. A significant increase in NMDAR1 immunoreactive protein was found in frontal cortex in the subacute group (P = .036) but not in the acute group (P = .580). Moreover, a significant increase in NMDAR1 was also observed in striatum in both acute (P = .025) and subacute groups (P = .023). However, no significant differences in NMDAR1 in hippocampal formation were observed in either acute or subacute group. The results suggest that an upregulation of NMDA receptor expression may be a consequence of glutamatergic dysfunction induced by METH. |
format | Text |
id | pubmed-2686235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-26862352009-05-28 Changes in Glutamate/NMDA Receptor Subunit 1 Expression in Rat Brain after Acute and Subacute Exposure to Methamphetamine Kerdsan, Walailuk Thanoi, Samur Nudmamud-Thanoi, Sutisa J Biomed Biotechnol Research Article Methamphetamine (METH) is a psychostimulant drug of abuse that produces long-term behavioral changes including behavioral sensitization, tolerance, and dependence. METH has been reported to induce neurotoxic effects in several areas of the brain via the dopaminergic system. Changes of dopamine function can induce malfunction of the glutamatergic system. Therefore, the aim of the present study was to examine the effects of METH administration on the expression of glutamate N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in frontal cortex, striatum, and hippocampal formation after acute and subacute exposure to METH by western blotting. Male Sprague-Dawley rats were injected intraperitoneally with a single dose of 8 mg/kg METH, 4 mg/kg/day METH for 14 days and saline in acute, subacute, and control groups, respectively. A significant increase in NMDAR1 immunoreactive protein was found in frontal cortex in the subacute group (P = .036) but not in the acute group (P = .580). Moreover, a significant increase in NMDAR1 was also observed in striatum in both acute (P = .025) and subacute groups (P = .023). However, no significant differences in NMDAR1 in hippocampal formation were observed in either acute or subacute group. The results suggest that an upregulation of NMDA receptor expression may be a consequence of glutamatergic dysfunction induced by METH. Hindawi Publishing Corporation 2009 2009-05-26 /pmc/articles/PMC2686235/ /pubmed/19478962 http://dx.doi.org/10.1155/2009/329631 Text en Copyright © 2009 Walailuk Kerdsan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kerdsan, Walailuk Thanoi, Samur Nudmamud-Thanoi, Sutisa Changes in Glutamate/NMDA Receptor Subunit 1 Expression in Rat Brain after Acute and Subacute Exposure to Methamphetamine |
title | Changes in Glutamate/NMDA Receptor Subunit 1 Expression in Rat Brain after Acute and Subacute Exposure to Methamphetamine |
title_full | Changes in Glutamate/NMDA Receptor Subunit 1 Expression in Rat Brain after Acute and Subacute Exposure to Methamphetamine |
title_fullStr | Changes in Glutamate/NMDA Receptor Subunit 1 Expression in Rat Brain after Acute and Subacute Exposure to Methamphetamine |
title_full_unstemmed | Changes in Glutamate/NMDA Receptor Subunit 1 Expression in Rat Brain after Acute and Subacute Exposure to Methamphetamine |
title_short | Changes in Glutamate/NMDA Receptor Subunit 1 Expression in Rat Brain after Acute and Subacute Exposure to Methamphetamine |
title_sort | changes in glutamate/nmda receptor subunit 1 expression in rat brain after acute and subacute exposure to methamphetamine |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2686235/ https://www.ncbi.nlm.nih.gov/pubmed/19478962 http://dx.doi.org/10.1155/2009/329631 |
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