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Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator

A putative spindle matrix has been hypothesized to mediate chromosome motion, but its existence and functionality remain controversial. In this report, we show that Megator (Mtor), the Drosophila melanogaster counterpart of the human nuclear pore complex protein translocated promoter region (Tpr), a...

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Autores principales: Lince-Faria, Mariana, Maffini, Stefano, Orr, Bernard, Ding, Yun, Cláudia Florindo, Sunkel, Claudio E., Tavares, Álvaro, Johansen, Jørgen, Johansen, Kristen M., Maiato, Helder
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2686406/
https://www.ncbi.nlm.nih.gov/pubmed/19273613
http://dx.doi.org/10.1083/jcb.200811012
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author Lince-Faria, Mariana
Maffini, Stefano
Orr, Bernard
Ding, Yun
Cláudia Florindo,
Sunkel, Claudio E.
Tavares, Álvaro
Johansen, Jørgen
Johansen, Kristen M.
Maiato, Helder
author_facet Lince-Faria, Mariana
Maffini, Stefano
Orr, Bernard
Ding, Yun
Cláudia Florindo,
Sunkel, Claudio E.
Tavares, Álvaro
Johansen, Jørgen
Johansen, Kristen M.
Maiato, Helder
author_sort Lince-Faria, Mariana
collection PubMed
description A putative spindle matrix has been hypothesized to mediate chromosome motion, but its existence and functionality remain controversial. In this report, we show that Megator (Mtor), the Drosophila melanogaster counterpart of the human nuclear pore complex protein translocated promoter region (Tpr), and the spindle assembly checkpoint (SAC) protein Mad2 form a conserved complex that localizes to a nuclear derived spindle matrix in living cells. Fluorescence recovery after photobleaching experiments supports that Mtor is retained around spindle microtubules, where it shows distinct dynamic properties. Mtor/Tpr promotes the recruitment of Mad2 and Mps1 but not Mad1 to unattached kinetochores (KTs), mediating normal mitotic duration and SAC response. At anaphase, Mtor plays a role in spindle elongation, thereby affecting normal chromosome movement. We propose that Mtor/Tpr functions as a spatial regulator of the SAC, which ensures the efficient recruitment of Mad2 to unattached KTs at the onset of mitosis and proper spindle maturation, whereas enrichment of Mad2 in a spindle matrix helps confine the action of a diffusible “wait anaphase” signal to the vicinity of the spindle.
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spelling pubmed-26864062009-09-09 Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator Lince-Faria, Mariana Maffini, Stefano Orr, Bernard Ding, Yun Cláudia Florindo, Sunkel, Claudio E. Tavares, Álvaro Johansen, Jørgen Johansen, Kristen M. Maiato, Helder J Cell Biol Research Articles A putative spindle matrix has been hypothesized to mediate chromosome motion, but its existence and functionality remain controversial. In this report, we show that Megator (Mtor), the Drosophila melanogaster counterpart of the human nuclear pore complex protein translocated promoter region (Tpr), and the spindle assembly checkpoint (SAC) protein Mad2 form a conserved complex that localizes to a nuclear derived spindle matrix in living cells. Fluorescence recovery after photobleaching experiments supports that Mtor is retained around spindle microtubules, where it shows distinct dynamic properties. Mtor/Tpr promotes the recruitment of Mad2 and Mps1 but not Mad1 to unattached kinetochores (KTs), mediating normal mitotic duration and SAC response. At anaphase, Mtor plays a role in spindle elongation, thereby affecting normal chromosome movement. We propose that Mtor/Tpr functions as a spatial regulator of the SAC, which ensures the efficient recruitment of Mad2 to unattached KTs at the onset of mitosis and proper spindle maturation, whereas enrichment of Mad2 in a spindle matrix helps confine the action of a diffusible “wait anaphase” signal to the vicinity of the spindle. The Rockefeller University Press 2009-03-09 /pmc/articles/PMC2686406/ /pubmed/19273613 http://dx.doi.org/10.1083/jcb.200811012 Text en © 2009 Lince-Faria et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Lince-Faria, Mariana
Maffini, Stefano
Orr, Bernard
Ding, Yun
Cláudia Florindo,
Sunkel, Claudio E.
Tavares, Álvaro
Johansen, Jørgen
Johansen, Kristen M.
Maiato, Helder
Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator
title Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator
title_full Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator
title_fullStr Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator
title_full_unstemmed Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator
title_short Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator
title_sort spatiotemporal control of mitosis by the conserved spindle matrix protein megator
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2686406/
https://www.ncbi.nlm.nih.gov/pubmed/19273613
http://dx.doi.org/10.1083/jcb.200811012
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