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A Casein Kinase 1 and PAR Proteins Regulate Asymmetry of a PIP(2) Synthesis Enzyme for Asymmetric Spindle Positioning
Spindle positioning is an essential feature of asymmetric cell division. The conserved PAR proteins together with heterotrimeric G proteins control spindle positioning in animal cells, but how these are linked is not known. In C. elegans, PAR protein activity leads to asymmetric spindle placement th...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2686839/ https://www.ncbi.nlm.nih.gov/pubmed/18694560 http://dx.doi.org/10.1016/j.devcel.2008.06.002 |
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author | Panbianco, Costanza Weinkove, David Zanin, Esther Jones, David Divecha, Nullin Gotta, Monica Ahringer, Julie |
author_facet | Panbianco, Costanza Weinkove, David Zanin, Esther Jones, David Divecha, Nullin Gotta, Monica Ahringer, Julie |
author_sort | Panbianco, Costanza |
collection | PubMed |
description | Spindle positioning is an essential feature of asymmetric cell division. The conserved PAR proteins together with heterotrimeric G proteins control spindle positioning in animal cells, but how these are linked is not known. In C. elegans, PAR protein activity leads to asymmetric spindle placement through cortical asymmetry of Gα regulators GPR-1/2. Here, we establish that the casein kinase 1 gamma CSNK-1 and a PIP(2) synthesis enzyme (PPK-1) transduce PAR polarity to asymmetric Gα regulation. PPK-1 is posteriorly enriched in the one-celled embryo through PAR and CSNK-1 activities. Loss of CSNK-1 causes uniformly high PPK-1 levels, high symmetric cortical levels of GPR-1/2 and LIN-5, and increased spindle pulling forces. In contrast, knockdown of ppk-1 leads to low GPR-1/2 levels and decreased spindle forces. Furthermore, loss of CSNK-1 leads to increased levels of PIP(2). We propose that asymmetric generation of PIP(2) by PPK-1 directs the posterior enrichment of GPR-1/2 and LIN-5, leading to posterior spindle displacement. |
format | Text |
id | pubmed-2686839 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-26868392009-05-28 A Casein Kinase 1 and PAR Proteins Regulate Asymmetry of a PIP(2) Synthesis Enzyme for Asymmetric Spindle Positioning Panbianco, Costanza Weinkove, David Zanin, Esther Jones, David Divecha, Nullin Gotta, Monica Ahringer, Julie Dev Cell Article Spindle positioning is an essential feature of asymmetric cell division. The conserved PAR proteins together with heterotrimeric G proteins control spindle positioning in animal cells, but how these are linked is not known. In C. elegans, PAR protein activity leads to asymmetric spindle placement through cortical asymmetry of Gα regulators GPR-1/2. Here, we establish that the casein kinase 1 gamma CSNK-1 and a PIP(2) synthesis enzyme (PPK-1) transduce PAR polarity to asymmetric Gα regulation. PPK-1 is posteriorly enriched in the one-celled embryo through PAR and CSNK-1 activities. Loss of CSNK-1 causes uniformly high PPK-1 levels, high symmetric cortical levels of GPR-1/2 and LIN-5, and increased spindle pulling forces. In contrast, knockdown of ppk-1 leads to low GPR-1/2 levels and decreased spindle forces. Furthermore, loss of CSNK-1 leads to increased levels of PIP(2). We propose that asymmetric generation of PIP(2) by PPK-1 directs the posterior enrichment of GPR-1/2 and LIN-5, leading to posterior spindle displacement. Cell Press 2008-08-12 /pmc/articles/PMC2686839/ /pubmed/18694560 http://dx.doi.org/10.1016/j.devcel.2008.06.002 Text en © 2008 ELL & Excerpta Medica. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Panbianco, Costanza Weinkove, David Zanin, Esther Jones, David Divecha, Nullin Gotta, Monica Ahringer, Julie A Casein Kinase 1 and PAR Proteins Regulate Asymmetry of a PIP(2) Synthesis Enzyme for Asymmetric Spindle Positioning |
title | A Casein Kinase 1 and PAR Proteins Regulate Asymmetry of a PIP(2) Synthesis Enzyme for Asymmetric Spindle Positioning |
title_full | A Casein Kinase 1 and PAR Proteins Regulate Asymmetry of a PIP(2) Synthesis Enzyme for Asymmetric Spindle Positioning |
title_fullStr | A Casein Kinase 1 and PAR Proteins Regulate Asymmetry of a PIP(2) Synthesis Enzyme for Asymmetric Spindle Positioning |
title_full_unstemmed | A Casein Kinase 1 and PAR Proteins Regulate Asymmetry of a PIP(2) Synthesis Enzyme for Asymmetric Spindle Positioning |
title_short | A Casein Kinase 1 and PAR Proteins Regulate Asymmetry of a PIP(2) Synthesis Enzyme for Asymmetric Spindle Positioning |
title_sort | casein kinase 1 and par proteins regulate asymmetry of a pip(2) synthesis enzyme for asymmetric spindle positioning |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2686839/ https://www.ncbi.nlm.nih.gov/pubmed/18694560 http://dx.doi.org/10.1016/j.devcel.2008.06.002 |
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