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Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics

BACKGROUND/AIMS: The androgen dehydroepiandrosterone (DHEA) attenuates allergic inflammatory airway reactions by down-regulating the Th2 response in mice. The purpose of this study was to investigate whether DHEA suppresses Th2 cytokine production in cultured peripheral blood mononuclear cells (PBMC...

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Autores principales: Choi, Inseon S., Cui, Yong, Koh, Young-Ah, Lee, Hyun-Chul, Cho, Yong-Bum, Won, Young-Ho
Formato: Texto
Lenguaje:English
Publicado: The Korean Association of Internal Medicine 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2687683/
https://www.ncbi.nlm.nih.gov/pubmed/19119254
http://dx.doi.org/10.3904/kjim.2008.23.4.176
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author Choi, Inseon S.
Cui, Yong
Koh, Young-Ah
Lee, Hyun-Chul
Cho, Yong-Bum
Won, Young-Ho
author_facet Choi, Inseon S.
Cui, Yong
Koh, Young-Ah
Lee, Hyun-Chul
Cho, Yong-Bum
Won, Young-Ho
author_sort Choi, Inseon S.
collection PubMed
description BACKGROUND/AIMS: The androgen dehydroepiandrosterone (DHEA) attenuates allergic inflammatory airway reactions by down-regulating the Th2 response in mice. The purpose of this study was to investigate whether DHEA suppresses Th2 cytokine production in cultured peripheral blood mononuclear cells (PBMCs) from asthmatic patients. METHODS: Sixty-one consecutive suspected asthmatic or non-asthmatic men underwent tests for asthma. PBMCs from each subject were cultured with and without DHEA (0.01~10 µM) for 48 h. The concentrations of interferon (IFN)-γ, interleukin (IL)-5, and IL-10 in the culture supernatant were measured via an enzyme-linked immunosorbent assay. RESULTS: In PBMCs from subjects exhibiting methacholine airway hyperresponsiveness (AHR), DHEA significantly suppressed IL-10, IL-5, and IFN-γ production in a dose-dependent manner (all p<0.001) and tended to increase the IFN-γ/IL-5 ratio (p=0.087). DHEA (10 µM) suppressed cytokine production to a greater degree in subjects with AHR compared with those without AHR (IL-5: 24.0±7.8% vs. 40.9±3.6%, p<0.01; IFN-γ: 29.7±7.0% vs. 54.5±5.1%, p<0.01). Cytokine suppression was significantly related to AHR, serum total IgE levels, and skin reactivity to house dust mites. CONCLUSIONS: DHEA suppressed both Th1 and Th2 responses, with a Th1 bias, and the degree of suppression was associated with the severity of AHR or atopy. Therefore, DHEA may be a useful therapy for asthma.
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spelling pubmed-26876832009-06-15 Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics Choi, Inseon S. Cui, Yong Koh, Young-Ah Lee, Hyun-Chul Cho, Yong-Bum Won, Young-Ho Korean J Intern Med Original Article BACKGROUND/AIMS: The androgen dehydroepiandrosterone (DHEA) attenuates allergic inflammatory airway reactions by down-regulating the Th2 response in mice. The purpose of this study was to investigate whether DHEA suppresses Th2 cytokine production in cultured peripheral blood mononuclear cells (PBMCs) from asthmatic patients. METHODS: Sixty-one consecutive suspected asthmatic or non-asthmatic men underwent tests for asthma. PBMCs from each subject were cultured with and without DHEA (0.01~10 µM) for 48 h. The concentrations of interferon (IFN)-γ, interleukin (IL)-5, and IL-10 in the culture supernatant were measured via an enzyme-linked immunosorbent assay. RESULTS: In PBMCs from subjects exhibiting methacholine airway hyperresponsiveness (AHR), DHEA significantly suppressed IL-10, IL-5, and IFN-γ production in a dose-dependent manner (all p<0.001) and tended to increase the IFN-γ/IL-5 ratio (p=0.087). DHEA (10 µM) suppressed cytokine production to a greater degree in subjects with AHR compared with those without AHR (IL-5: 24.0±7.8% vs. 40.9±3.6%, p<0.01; IFN-γ: 29.7±7.0% vs. 54.5±5.1%, p<0.01). Cytokine suppression was significantly related to AHR, serum total IgE levels, and skin reactivity to house dust mites. CONCLUSIONS: DHEA suppressed both Th1 and Th2 responses, with a Th1 bias, and the degree of suppression was associated with the severity of AHR or atopy. Therefore, DHEA may be a useful therapy for asthma. The Korean Association of Internal Medicine 2008-12 2008-12-30 /pmc/articles/PMC2687683/ /pubmed/19119254 http://dx.doi.org/10.3904/kjim.2008.23.4.176 Text en Copyright © 2008 The Korean Association of Internal Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Choi, Inseon S.
Cui, Yong
Koh, Young-Ah
Lee, Hyun-Chul
Cho, Yong-Bum
Won, Young-Ho
Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics
title Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics
title_full Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics
title_fullStr Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics
title_full_unstemmed Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics
title_short Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics
title_sort effects of dehydroepiandrosterone on th2 cytokine production in peripheral blood mononuclear cells from asthmatics
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2687683/
https://www.ncbi.nlm.nih.gov/pubmed/19119254
http://dx.doi.org/10.3904/kjim.2008.23.4.176
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