Expression of p16 in Conjunctival Intraepithelial Neoplasia Does Not Correlate with HPV-Infection

The aim of our study was to identify the frequency of expression of p16(INK4a) (CDKN2A) and HPV (human papilloma virus) in different grades of conjunctival intraepithelial neoplasia (CIN). Twelve specimens including CIN I (2), II (3), III (5), and CIN with beginning invasion (2), as well as 15 contr...

Descripción completa

Detalles Bibliográficos
Autores principales: Auw-Haedrich, Claudia, Martin, Gottfried, Spelsberg, Helga, Sundmacher, Rainer, Freudenberg, Nikolaus, Maier, Philip, Reinhard, Thomas
Formato: Texto
Lenguaje:English
Publicado: Bentham Science Publishers Ltd. 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2687927/
https://www.ncbi.nlm.nih.gov/pubmed/19516893
http://dx.doi.org/10.2174/1874364100802010048
_version_ 1782167622802997248
author Auw-Haedrich, Claudia
Martin, Gottfried
Spelsberg, Helga
Sundmacher, Rainer
Freudenberg, Nikolaus
Maier, Philip
Reinhard, Thomas
author_facet Auw-Haedrich, Claudia
Martin, Gottfried
Spelsberg, Helga
Sundmacher, Rainer
Freudenberg, Nikolaus
Maier, Philip
Reinhard, Thomas
author_sort Auw-Haedrich, Claudia
collection PubMed
description The aim of our study was to identify the frequency of expression of p16(INK4a) (CDKN2A) and HPV (human papilloma virus) in different grades of conjunctival intraepithelial neoplasia (CIN). Twelve specimens including CIN I (2), II (3), III (5), and CIN with beginning invasion (2), as well as 15 control specimens, were stained with antibodies against p16(INK4a) and MIB1. The presence of HPV was examined by PCR. p16 as well as MIB1 were significantly elevated in CIN compared to control specimens (p<0.01) without correlation with the differentiation grade. Only two cases with CIN grade 3 contained HPV 16. As few control specimens also showed increased p16(INK4a) expression, p16(INK4a) seems not to be a very reliable marker for the exact determination of CIN. It could serve as an additional diagnostic tool besides the morphological characterization. Our study suggested that p16(INK4a) elevation is not associated with HPV infection.
format Text
id pubmed-2687927
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Bentham Science Publishers Ltd.
record_format MEDLINE/PubMed
spelling pubmed-26879272009-06-09 Expression of p16 in Conjunctival Intraepithelial Neoplasia Does Not Correlate with HPV-Infection Auw-Haedrich, Claudia Martin, Gottfried Spelsberg, Helga Sundmacher, Rainer Freudenberg, Nikolaus Maier, Philip Reinhard, Thomas Open Ophthalmol J Article The aim of our study was to identify the frequency of expression of p16(INK4a) (CDKN2A) and HPV (human papilloma virus) in different grades of conjunctival intraepithelial neoplasia (CIN). Twelve specimens including CIN I (2), II (3), III (5), and CIN with beginning invasion (2), as well as 15 control specimens, were stained with antibodies against p16(INK4a) and MIB1. The presence of HPV was examined by PCR. p16 as well as MIB1 were significantly elevated in CIN compared to control specimens (p<0.01) without correlation with the differentiation grade. Only two cases with CIN grade 3 contained HPV 16. As few control specimens also showed increased p16(INK4a) expression, p16(INK4a) seems not to be a very reliable marker for the exact determination of CIN. It could serve as an additional diagnostic tool besides the morphological characterization. Our study suggested that p16(INK4a) elevation is not associated with HPV infection. Bentham Science Publishers Ltd. 2008-03-28 /pmc/articles/PMC2687927/ /pubmed/19516893 http://dx.doi.org/10.2174/1874364100802010048 Text en 2008 Bentham Science Publishers Ltd. http://creativecommons.org/licenses/by/2.5/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/) which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Auw-Haedrich, Claudia
Martin, Gottfried
Spelsberg, Helga
Sundmacher, Rainer
Freudenberg, Nikolaus
Maier, Philip
Reinhard, Thomas
Expression of p16 in Conjunctival Intraepithelial Neoplasia Does Not Correlate with HPV-Infection
title Expression of p16 in Conjunctival Intraepithelial Neoplasia Does Not Correlate with HPV-Infection
title_full Expression of p16 in Conjunctival Intraepithelial Neoplasia Does Not Correlate with HPV-Infection
title_fullStr Expression of p16 in Conjunctival Intraepithelial Neoplasia Does Not Correlate with HPV-Infection
title_full_unstemmed Expression of p16 in Conjunctival Intraepithelial Neoplasia Does Not Correlate with HPV-Infection
title_short Expression of p16 in Conjunctival Intraepithelial Neoplasia Does Not Correlate with HPV-Infection
title_sort expression of p16 in conjunctival intraepithelial neoplasia does not correlate with hpv-infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2687927/
https://www.ncbi.nlm.nih.gov/pubmed/19516893
http://dx.doi.org/10.2174/1874364100802010048
work_keys_str_mv AT auwhaedrichclaudia expressionofp16inconjunctivalintraepithelialneoplasiadoesnotcorrelatewithhpvinfection
AT martingottfried expressionofp16inconjunctivalintraepithelialneoplasiadoesnotcorrelatewithhpvinfection
AT spelsberghelga expressionofp16inconjunctivalintraepithelialneoplasiadoesnotcorrelatewithhpvinfection
AT sundmacherrainer expressionofp16inconjunctivalintraepithelialneoplasiadoesnotcorrelatewithhpvinfection
AT freudenbergnikolaus expressionofp16inconjunctivalintraepithelialneoplasiadoesnotcorrelatewithhpvinfection
AT maierphilip expressionofp16inconjunctivalintraepithelialneoplasiadoesnotcorrelatewithhpvinfection
AT reinhardthomas expressionofp16inconjunctivalintraepithelialneoplasiadoesnotcorrelatewithhpvinfection

Ejemplares similares