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Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes

INTRODUCTION: In the present study, we investigated the ability of microparticles isolated from synovial fluids from patients with rheumatoid arthritis or osteoarthritis to induce the synthesis and release of key cytokines of B-lymphocyte modulation such as B cell-activating factor, thymic stroma ly...

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Autores principales: Messer, Laurent, Alsaleh, Ghada, Freyssinet, Jean-Marie, Zobairi, Fatiha, Leray, Isabelle, Gottenberg, Jacques-Eric, Sibilia, Jean, Toti-Orfanoudakis, Florence, Wachsmann, Dominique
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688187/
https://www.ncbi.nlm.nih.gov/pubmed/19291304
http://dx.doi.org/10.1186/ar2648
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author Messer, Laurent
Alsaleh, Ghada
Freyssinet, Jean-Marie
Zobairi, Fatiha
Leray, Isabelle
Gottenberg, Jacques-Eric
Sibilia, Jean
Toti-Orfanoudakis, Florence
Wachsmann, Dominique
author_facet Messer, Laurent
Alsaleh, Ghada
Freyssinet, Jean-Marie
Zobairi, Fatiha
Leray, Isabelle
Gottenberg, Jacques-Eric
Sibilia, Jean
Toti-Orfanoudakis, Florence
Wachsmann, Dominique
author_sort Messer, Laurent
collection PubMed
description INTRODUCTION: In the present study, we investigated the ability of microparticles isolated from synovial fluids from patients with rheumatoid arthritis or osteoarthritis to induce the synthesis and release of key cytokines of B-lymphocyte modulation such as B cell-activating factor, thymic stroma lymphopoietin, and secretory leukocyte protease inhibitor by rheumatoid fibroblast-like synoviocytes. METHODS: Microparticles were analyzed in synovial fluids from patients with rheumatoid arthritis, osteoarthritis, microcristalline arthritis, and reactive arthritis. In addition, microparticle release after activation from various cell lines (CEM lymphocyte and THP-1 cells) was assessed. Microparticles were isolated by differential centrifugation, and quantitative determinations were carried out by prothrombinase assay after capture on immobilized annexin V. B cell-activating factor, thymic stroma lymphopoietin, and secretory leukocyte protease inhibitor release was evaluated by enzyme-linked immunosorbent assay. RESULTS: Microparticles isolated from synovial fluids obtained from rheumatoid arthritis and osteoarthritis patients or microparticles derived from activated THP-1 cells were able to induce B cell-activating factor, thymic stroma lymphopoietin, and secretory leukocyte protease inhibitor release by rheumatoid arthritis fibroblast-like synoviocytes. Conversely, CEM-lymphocytes-derived microparticles generated by treatment with a combination of PHA, PMA and Adt-D did not promote the release of B cell-activating factor but favored the secretion of thymic stroma lymphopoietin and secretory leukocyte protease inhibitor by rheumatoid arthritis fibrobast-like synoviocytes. However, microparticles isolated from actinomycin D-treated CEM lymphocytes were not able to induce B cell-activating factor, thymic stroma lymphopoietin, or secretory leukocyte protease inhibitor release, indicating that microparticles derived from apoptotic T cells do not function as effectors in B-cell activation. CONCLUSIONS: These results demonstrate that microparticles are signalling structures that may act as specific conveyors in the triggered induction and amplification of autoimmunity. This study also indicates that microparticles have differential effects in the crosstalk between B lymphocytes and target cells of autoimmunity regarding the parental cells from which they derive.
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spelling pubmed-26881872009-05-29 Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes Messer, Laurent Alsaleh, Ghada Freyssinet, Jean-Marie Zobairi, Fatiha Leray, Isabelle Gottenberg, Jacques-Eric Sibilia, Jean Toti-Orfanoudakis, Florence Wachsmann, Dominique Arthritis Res Ther Research Article INTRODUCTION: In the present study, we investigated the ability of microparticles isolated from synovial fluids from patients with rheumatoid arthritis or osteoarthritis to induce the synthesis and release of key cytokines of B-lymphocyte modulation such as B cell-activating factor, thymic stroma lymphopoietin, and secretory leukocyte protease inhibitor by rheumatoid fibroblast-like synoviocytes. METHODS: Microparticles were analyzed in synovial fluids from patients with rheumatoid arthritis, osteoarthritis, microcristalline arthritis, and reactive arthritis. In addition, microparticle release after activation from various cell lines (CEM lymphocyte and THP-1 cells) was assessed. Microparticles were isolated by differential centrifugation, and quantitative determinations were carried out by prothrombinase assay after capture on immobilized annexin V. B cell-activating factor, thymic stroma lymphopoietin, and secretory leukocyte protease inhibitor release was evaluated by enzyme-linked immunosorbent assay. RESULTS: Microparticles isolated from synovial fluids obtained from rheumatoid arthritis and osteoarthritis patients or microparticles derived from activated THP-1 cells were able to induce B cell-activating factor, thymic stroma lymphopoietin, and secretory leukocyte protease inhibitor release by rheumatoid arthritis fibroblast-like synoviocytes. Conversely, CEM-lymphocytes-derived microparticles generated by treatment with a combination of PHA, PMA and Adt-D did not promote the release of B cell-activating factor but favored the secretion of thymic stroma lymphopoietin and secretory leukocyte protease inhibitor by rheumatoid arthritis fibrobast-like synoviocytes. However, microparticles isolated from actinomycin D-treated CEM lymphocytes were not able to induce B cell-activating factor, thymic stroma lymphopoietin, or secretory leukocyte protease inhibitor release, indicating that microparticles derived from apoptotic T cells do not function as effectors in B-cell activation. CONCLUSIONS: These results demonstrate that microparticles are signalling structures that may act as specific conveyors in the triggered induction and amplification of autoimmunity. This study also indicates that microparticles have differential effects in the crosstalk between B lymphocytes and target cells of autoimmunity regarding the parental cells from which they derive. BioMed Central 2009 2009-03-16 /pmc/articles/PMC2688187/ /pubmed/19291304 http://dx.doi.org/10.1186/ar2648 Text en Copyright © 2009 Messer et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Messer, Laurent
Alsaleh, Ghada
Freyssinet, Jean-Marie
Zobairi, Fatiha
Leray, Isabelle
Gottenberg, Jacques-Eric
Sibilia, Jean
Toti-Orfanoudakis, Florence
Wachsmann, Dominique
Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes
title Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes
title_full Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes
title_fullStr Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes
title_full_unstemmed Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes
title_short Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes
title_sort microparticle-induced release of b-lymphocyte regulators by rheumatoid synoviocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688187/
https://www.ncbi.nlm.nih.gov/pubmed/19291304
http://dx.doi.org/10.1186/ar2648
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