Fast genomic μChIP-chip from 1,000 cells

Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We...

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Detalles Bibliográficos
Autores principales: Dahl, John Arne, Reiner, Andrew H, Collas, Philippe
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688267/
https://www.ncbi.nlm.nih.gov/pubmed/19208222
http://dx.doi.org/10.1186/gb-2009-10-2-r13
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author Dahl, John Arne
Reiner, Andrew H
Collas, Philippe
author_facet Dahl, John Arne
Reiner, Andrew H
Collas, Philippe
author_sort Dahl, John Arne
collection PubMed
description Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (μChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. μChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed.
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spelling pubmed-26882672009-05-29 Fast genomic μChIP-chip from 1,000 cells Dahl, John Arne Reiner, Andrew H Collas, Philippe Genome Biol Method Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (μChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. μChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed. BioMed Central 2009 2009-02-10 /pmc/articles/PMC2688267/ /pubmed/19208222 http://dx.doi.org/10.1186/gb-2009-10-2-r13 Text en Copyright © 2009 Dahl et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Dahl, John Arne
Reiner, Andrew H
Collas, Philippe
Fast genomic μChIP-chip from 1,000 cells
title Fast genomic μChIP-chip from 1,000 cells
title_full Fast genomic μChIP-chip from 1,000 cells
title_fullStr Fast genomic μChIP-chip from 1,000 cells
title_full_unstemmed Fast genomic μChIP-chip from 1,000 cells
title_short Fast genomic μChIP-chip from 1,000 cells
title_sort fast genomic μchip-chip from 1,000 cells
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688267/
https://www.ncbi.nlm.nih.gov/pubmed/19208222
http://dx.doi.org/10.1186/gb-2009-10-2-r13
work_keys_str_mv AT dahljohnarne fastgenomicmchipchipfrom1000cells
AT reinerandrewh fastgenomicmchipchipfrom1000cells
AT collasphilippe fastgenomicmchipchipfrom1000cells

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