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DICER1 and PRKRA in Colon Adenocarcinoma

Differential microRNA expression in colon adenocarcinoma (CA) was previously reported. MicroRNA biogenesis and function requires a set of proteins designated as the microRNA machinery, which includes DICER1 and PRKRA. Loss of heterozygosity at 14q32.13 DICER1 locus was detected in up to 60% of CA ca...

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Autores principales: Chiosea, S., Acquafondata, M., Luo, J., Kuan, SF., Seethala, RR.
Formato: Texto
Lenguaje:English
Publicado: Libertas Academica 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688376/
https://www.ncbi.nlm.nih.gov/pubmed/19578509
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author Chiosea, S.
Acquafondata, M.
Luo, J.
Kuan, SF.
Seethala, RR.
author_facet Chiosea, S.
Acquafondata, M.
Luo, J.
Kuan, SF.
Seethala, RR.
author_sort Chiosea, S.
collection PubMed
description Differential microRNA expression in colon adenocarcinoma (CA) was previously reported. MicroRNA biogenesis and function requires a set of proteins designated as the microRNA machinery, which includes DICER1 and PRKRA. Loss of heterozygosity at 14q32.13 DICER1 locus was detected in up to 60% of CA cases. The in silico gene array analysis of CA showed down-regulation of DICER1 and an up-regulation of PRKRA. Immunohistochemically, DICER1 expression was abnormal in 65% of CA (95 of 147 cases). PRKRA was deregulated in 70% of CA (32 of 46 cases). Expression of DICER1 and PRKRA was correlated with clinicopathologic features of CA. DICER1 up-regulation was seen more commonly in women. Only 10 of 46 cases immunostained for both DICER1 and PRKRA showed normal levels of both DICER1 and PRKRA. Microsatellite status of 32 cases was determined. Microsatellite instable cases showed DICER1 up-regulation more commonly when compared to microsatellite stable cases; however, this trend was not statistically significant. Abnormal DICER1 and/or PRKRA expression might explain the observed changes in microRNA profile. The status of the endogenous DICER1 and PRKRA in CA may help to predict the response to future RNA interference-based therapy.
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spelling pubmed-26883762009-07-01 DICER1 and PRKRA in Colon Adenocarcinoma Chiosea, S. Acquafondata, M. Luo, J. Kuan, SF. Seethala, RR. Biomark Insights Original Research Differential microRNA expression in colon adenocarcinoma (CA) was previously reported. MicroRNA biogenesis and function requires a set of proteins designated as the microRNA machinery, which includes DICER1 and PRKRA. Loss of heterozygosity at 14q32.13 DICER1 locus was detected in up to 60% of CA cases. The in silico gene array analysis of CA showed down-regulation of DICER1 and an up-regulation of PRKRA. Immunohistochemically, DICER1 expression was abnormal in 65% of CA (95 of 147 cases). PRKRA was deregulated in 70% of CA (32 of 46 cases). Expression of DICER1 and PRKRA was correlated with clinicopathologic features of CA. DICER1 up-regulation was seen more commonly in women. Only 10 of 46 cases immunostained for both DICER1 and PRKRA showed normal levels of both DICER1 and PRKRA. Microsatellite status of 32 cases was determined. Microsatellite instable cases showed DICER1 up-regulation more commonly when compared to microsatellite stable cases; however, this trend was not statistically significant. Abnormal DICER1 and/or PRKRA expression might explain the observed changes in microRNA profile. The status of the endogenous DICER1 and PRKRA in CA may help to predict the response to future RNA interference-based therapy. Libertas Academica 2008-04-28 /pmc/articles/PMC2688376/ /pubmed/19578509 Text en © 2008 by the authors http://creativecommons.org/licenses/by/3.0 This article is published under the Creative Commons Attribution By licence. For further information go to: http://creativecommons.org/licenses/by/3.0. (http://creativecommons.org/licenses/by/3.0)
spellingShingle Original Research
Chiosea, S.
Acquafondata, M.
Luo, J.
Kuan, SF.
Seethala, RR.
DICER1 and PRKRA in Colon Adenocarcinoma
title DICER1 and PRKRA in Colon Adenocarcinoma
title_full DICER1 and PRKRA in Colon Adenocarcinoma
title_fullStr DICER1 and PRKRA in Colon Adenocarcinoma
title_full_unstemmed DICER1 and PRKRA in Colon Adenocarcinoma
title_short DICER1 and PRKRA in Colon Adenocarcinoma
title_sort dicer1 and prkra in colon adenocarcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688376/
https://www.ncbi.nlm.nih.gov/pubmed/19578509
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