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Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli
Bacteria are simple and cost effective hosts for producing recombinant proteins. However, their physiological features may limit their use for obtaining in native form proteins of some specific structural classes, such as for instance polypeptides that undergo extensive post-translational modificati...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2689190/ https://www.ncbi.nlm.nih.gov/pubmed/19442264 http://dx.doi.org/10.1186/1475-2859-8-26 |